Gene Editing Techniques
1. Gene Editing – General Background
Gene editing entails inserting, deleting and replace the DNA in a genome of an organism utilizing engineered nucleases. Sections of the natural gene are replaced and completed using a synthetic DNA chain. Also, the natural repair process gets rid of the mismatches and gaps in the DNA. On the other hand, gene disruption is a technique by which the DNA fragment is utilized to replace a genome sequence with a chosen marker gene like the kanavanine resistance. DNA –binding proteins are proteins that consist of DNA-binding domains and hence have affinity for single and ...