Cholera Toxin Enhances Vaccine-Induced Protection against Mycobacterium Tuberculosis Challenge in Mice
This experiment is an animal experiment made in accordance with the basic guideline the UK Animal Act 1986. Methods use was by collecting mice of 6-8wks. BCG Pasteur vaccine administered. Others vaccines are MVA85A and cholera toxin. Intradermal agents administered are the anti-IL-1A blocking antibody and the isotype IgG2a. Animals were then challenge with Biaera AeroMP aerosol nebulizer at a targeted pressure and dose. Organ harvest and stimulation for immunogenicity was then done. The lungs and spleen are the organs harvested aseptically. The organs were then processed with different steps such as mincing and digestion with DNase/collagenases. The cells collected ...