Determining the size of gene fragments
1) Alul restriction enzyme cuts the protease gene at restriction sites with base position 62 and position 1149 respectively. Considering the fact that the gene has 1216 base pairs, three possible fragments are generated with Alul restriction digestion.
The first digestion fragment length ranges from base pair 1 to base pair 62= 61 base pairs.
The second fragment length ranges from base pair 62 to base pair 1149 in length=1087 base pairs
The third fragment ranges from base pair 1216 to base pair 1149 in length= 67 base pairs.
2) Sa/l restriction enzyme cuts the protease gene at ...