Compare and Contrast On IPS Papers
All the four papers Takahashi-miPS, Takahashi-hiPS, Park-hiPS and Yu-hiPS respectively induced the four genes (OCT4, SOX2, KLF4 and MYC) and reprogramming the differentiated somatic cells to produce IPS pluripotency cells. In the first paper, the authors found a combination of factors to make the IPS cells. The authors selected the 24 genes for factors that induce pluripotency in somatic cells. Afterwards, all the 24 genes were inserted into the mouse embryonic fibroblast (MEFs) by using retroviral transduction. The cells were cultured on STO feeder cells in ES cell medium containing G418. The authors obtained 24 colonies, and then the authors ...