I employed various avenues of tracking down information concerning my haplogroup and what it meant to be a member of my haplogroup. First, I opened the link given by the Professor to determine the various haplogroups, the HVS-1 motifs, the 73 status and the coding-region mutations. I studied each of the haplogroups and their corresponding HVS-1 motifs, their status and their coding-region mutations. I examined the transitions of the reference sequences basing on the indicated explicit HVS-1 positions. I identified a selection of the given coding-region mutations shown by the RFLP analysis. From my report, I aligned my sequence and recorded the length of the sequence. I then identified my nucleotides position, which was 15994 to 407 and identified all my mutations.
After identifying my mutations, I aligned and recorded their positions and their states including A, G, C and T to identify my haplotype. My mutations were 16181 C, 16182C, 16183 C, 16189 C, 16213 A, 16217 C, 16261 T, 6282 T, 6294 T, 16519 C, 73 G, 263 G, 309 deletion C and 310 insertion C. I arranged these mutations accordingly to identify the exact pattern of my haplotype, which would lead to my haplogroup. I then searched through the second website with the aligned mutations to determine the exact sequence and orientation of my genes and mutations. I put them in a distinct order that would separate the deletion, the insertion, the duplication and the translocation of the genes ("B2 gif"). After I had aligned these genes and their mutations, I realized that I fall in group B4 because my mutation was 189. I then sourced from the second website to align and check the purpose of my group.
The second website had important information concerning Mitochondrial DNA. There were various haplogroups but since I fall in group B4, I searched the haplogroups under B. the haplogroup B had various tree diagrams that indicated the type and sequence of all the groups under the haplogroup. There were five trees indicating the sequence of the haplogroups and haplotypes in the mitochondrial DNA. I found out various tree diagrams illustrating the various sequences under haplogroup B. from the information on the charts of the haplogroups I realized that every individual has their haplogroups that is distinct in their areas of origin. For instance, being in the haplogroup B meant that members of that group were spread throughout the Americas, Asia, and Melanesia. That is; it is a possible ancient group where individuals of the later haplogroups invaded the territories.
I also found out the various methods the haplogroups were typified. They were typified by the later differences from the CRS of 73, 263, 750, 1438, 2706, 4769, 7028, 8860, 11719, 1476 and 15326 ("Haplogroup motifs"). I followed the structure and sequence of the typified haplogroups to determine whether my group and its mutations were typified. I examined all the typified mutations and realized that a 9-base deletion occurred at the 8281-8289 mutation and CRS. The deletion occurred in other low frequencies in various haplogroups including T, L, I, D and C. the various deletions and insertions occurred in different areas including 309-315, 522-523 and 16182-16193 ("B1 gif"). Instability occurred in the region 16159. This information fully led to the haplogroup B-tree where I identified various mutation areas and groups that were compatible and related to my group, which is B4.
Works Cited
"B1 gif." Ian Logan. N.p., n.d. Web. 5 Dec. 2014.
"B2 gif." Ian Logan. N.p., n.d. Web. 5 Dec. 2014.
"Haplogroup motifs." University of Glasgow :: Subjects AZ :: Statistics. N.p., n.d. Web. 5 Dec. 2014.
