Question: what does this tell you about this fragment?
The illustration is a 3D structure of E2F4-DNA-DP2 complex. the green fragment is the transcription factor E2F4 protein and it also equates to one out of the four chains that the complete protein has
The fragment functions as a bride between DP-2 and the sequence of DNA.
The fragment has the potential of being involved in the process of transcription like functioning as a regulator factor in the cell cycle.
Can you think of a Reason why this stretch is so homologous?
The Yellow fragment corresponds to the DNA-binding domain which is a high homologous sequence of fragment between the E2F series. this portion determines serves as the functional unit of the molecul and defines the main purpose of the protein.
E2F4 protein possesses many domains like the transactivation domain which is highly rich in amino acids that are acidic. it also has a dimerization domain that specifies the interraction with the transcription factor protein known as DP-2.
Complexing
The molecular weight is 7.64 kDa. this weight is < 10kda and as such allows it to resist detection by the immune response leading to unsuccessful formation of antibodies. This translates to the fact that the protein mmust be couples with another molecule which is large in order to for an immune response to be produced
What does this tell you about this Fragment? can you think of two functions of this part of the protein.
It provides the information that the Chain A fragment is in close proximity to DNA i.e. DNA-Binding Domain
It also tells us that Chain A is biochemically of the protein class i.e. Transcription Factor E2F-4
This protein binds to the tumor supressor proteins pRB, p107 and p130.
Can you think of a reson why this stretch is so homologous?
The reason is because they are associated with DNA and they also interract with it.
Identity
The E2F4 proteins have quite a number of regions that have remain preserved throughout the process of evolution. The region has portion that binds to DNA, a region that causes dimerization which specifies the association with the transcription roteins (DP), and also a region which leads to transactivation which is preponderant in acidic amino acids and a domain which associated with the tumor supressor protein that is inserted in the region of transactivation.
