Part 1. Absorbance Spectra
The SpectroVis spectrophotometer cable was plugged into the LabQuest. The spectrophotometer was then calibrated by selecting the corresponding command from the menu. After the lamp has been warmed up, the cuvette filled with deionized water was put into the device, and the calibration was finished. Then, the cuvette with the red solution was wiped and inserted into the sample compartment with its clear face facing the arrow. The spectrum of the solution was obtained (using Start and Stop buttons). The results were saved and hidden using the File Cabinet. The cuvette with the red solution was removed from the spectrophotometer. The same steps were repeated to obtain the absorbance spectrum of the blue solution. The cable was unplugged from the LabQuest. Lastly, the experimental data was transferred to the flash drive.
Part 2. Absorbance vs. Concentration
Four 10.00 mL samples of diluted Red solution were prepared. The needed volume of the 0.5 M stock solution was transferred to the graduated cylinder, and then, the necessary amount of water was added to reach 10.00 mL total. The solution was thoroughly mixed and transferred to the test tube. The procedure was repeated for all the samples. The data on the samples is shown in the table:
The spectrophotometer was plugged into the LabQuest and recalibrated following the same procedure as in Part 1. After the calibration has been completed, the Mode of the device was changed to Concentration. The wavelength was set to 493, and the "reference blank" cuvette was prepared. It was emptied and rinsed twice with Trial 1 Red solution, it was filled with the same solution and placed in the spectrophotometer. The Start button was selected and then Keep button was selected, and the concentration of the sample was entered into the corresponding field. After the data point had been taken, the cuvette was emptied, rinsed, and filled with Trial 2 Solution. The data obtaining procedure was repeated for all the samples. After all the samples have been investigated, the spectrometer cable was unplugged, and the data transferred to flash drive. Lastly, the cuvettes were emptied into the waste container and washed with deionized water and the lab bench was cleaned with a paper towel.
