Introduction
The SNAP-25 is the synaptosomal-associated protein 25 which generated different primary and secondary effects on animals and human being including death. The most important symptoms (Huang, 2004) of the SNAP-25 are:
■ Attention deficit hyperactivity disorder: This affects the attention, excessive activity and difficulties in control behavior. Extreme situations may cause death.
■ RS362584 polymorphism in genes causing neuroticism in living beings, including humans. The neuroticism causes anxiety, fear, worry and frustration in individuals. In extreme cases, its causes the death.
The SNAP-25 biologically interrupts and destroys links and the structure of the ribonucleic acid of the cells.
In a laboratory analysis, the degradation of SNAP-25 will cause death if it is degraded to less than 25%.
The Cytochrome C is a protein that affects the mitochondrial structures of the cells of human beings. The Cytochrome can have an electron transport chain causing simultaneous oxidation and reduction process, without binding oxygen. The cytochrome c can cause apoptosis, that is, the process of killing cells due to an infection of deoxyribonucleic acids (Duregotti, 2015).
In a laboratory analysis, the cytochrome C may cause death in human beings if cytochrome C falls below two units per minute.
Method
The laboratory consists of the determination of the SNAP-25 levels and Cytochrome-C rate inside the blood sample of the analyzed individual, which was the subject of poisoning (PoisonOrg, 2016). A comparison, between the measured test and the theoretical framework of each protein, gives evidence to the analyst to determine which the cause of death of the individual was.
Each protein must be plotted to visualize the change in the conditions of each protein. A decreasing trend of level and rate respectively will give to the analyst enough evidence to determine what the cause of the death is: food or drink or a combination of both.
Results
Figure 1 shows the plot of Table 1 with a straight trend line
Figure 1 Food added (grams) Vs. SNAP-25
The trend line has a first-grade equation with the following expression:
Y = -0.712x + 103.82
X is the food added in grams and Y the SNAP-25 remaining in percentage. According to evidence, 25 grams of food was added having the following result:
Y = -0.712*(25) + 103.82
Y = +86.02%
The calculation shows that it remains 86.02% of SNAP-25.
The trend line has a second-grade equation with the following expression:
Y = 0,0015x2 - 0,2227x + 8,2332
X is the drink added in milliliters and Y the rate in units per minute. According to evidence, 50 milliliters of drink was added having the following result:
Y = 0,0015*(50)2 - 0,2227*(50) + 8,2332
Y = +0.85
The calculation shows that it the rate is 0.85 units per minute.
Discussion
The previous results gave the following results:
■ 86.02% of remaining SNAP-25
■ 0.85 units per minute of Cytochrome C
With the results of SNAP-25, there is no possibility that the individual is death by the food. The result of Cytochrome C gives 100% possibility that the individual is death by the drink.
The low rate per minute of Cytochrome C affected the normal functioning of the mitochondrial structures of cells in the individual. The mitochondria are the responsible to transform the energy into the cells, without the energy transformation, the cell does not have the possibility to survive in the long term causing malfunctioning of tissues and organs.
List of References
Duregotti, E., 2015. Mitochondrial alarmins released by degenerating motor axon terminals activate perisynaptic Schwann cells. Proc Natl Acad Sci U S A., 112(5), pp. E497-E505.
Huang, P., 2004. A study on the expression of Snap-25 mRNA in rat poisoned by tetramine. Sichuan Da Xue Xue Bao Yi Xue Ban, 35(5), p. 644.
PoisonOrg, 2016. Common and Dangerous Poisons. [Online] Available at: http://www.poison.org/common-and-dangerous-poisons[Accessed: 09 January 2017].
