According to Shortliffe and Cimino (2006), Bioinformatics enables students understand the processes involved in biotic systems. Biotic systems support in the study of how complex social systems interact by accumulating information, forming restrained behavior and maintaining prebiotic evolution models. Shortliffe and Cimino assert that developing this database we need to develop and design complex interfaces that researchers use to access the available data, revise it and then submit the data (2006). Bioinformatics helps to study, analyze and interpret data that involve amino acid and nucleotide sequence. Using of the computer has also improved the study of bioinformatics hence enabling the study of complex data in the social systems.
According to Shortliffe and Cimino (2006), Didexy is a usual DNA nucleotide used to sequence DNA. The second carbon on a Didexy does not have an oxygen atom, hence the name deoxy’d. Sanger’s sequence works by adding a DNA polymerase nucleotide in the fifth to third direction. Shortliffe and Cimino confirm that the fifth end hooks on the third hydroxyl group (2006). Dntps lack third carbon hydroxyl because the hydroxyl had removed the oxygen atom. The third carbon has H.
Therefore, extra nucleotides cannot add to the DNA polymerase. Shortliffe and Camino (2006) confirm that the four unique ddNTP tag on different fluorescent molecules. The DNA polymerase adds to the sequence and then synthesizes DNA strands that sequenced. The strand elongation stops when ddNTP incorporates in the sequence. The strand has different lengths depending on the amount of nucleotides incorporated in the sequence. Shortliffe and Camino confirms that color differentiates fluoresce in the sequence (2006). The strand moves through a gel after electrophoresis activates the strand. The length of the strand determines the speed of the strand such that the shortest strand moves faster than the longer strand. Therefore, a red strand shows that the stand has two nucleotides; yellow strand shows the strand has three nucleotides, and a red strand shows the strand has four nucleotides.
Sanger dideoxy diagram
It is a SOD1 form. This is because the SOD1 bind the zinc and copper ions to destroy the superoxide. The soluble cytoplasmic converts superoxide to hydrogen peroxide and molecular oxygen, making them have the difference in their genes. There are differences in the SOD1 and mutation form:
1. SOD1 genes have deleted cells while mutants have full cells.
2. SOD1 genes do not have G93A cells. Mutants have the G93A cells.
The difference in the SOD1 and mutant gene is due to the alignment in the nucleotides. The mutant nucleotides in the mutant cells have undergone double DNA alignment that has improved its nucleotides. The deleted gene in the SOD1 genes makes the DNA of the mutant cell different with the SOD1 cell. The G93A gene in the mutant cell brings he difference in the SOD1 genes and mutant cells.
Once I express the vector, I found out that the amino acid aligns itself according to the length of the strand. The longer strand moves slower than the strand with a shorter strand. Therefore, the nucleotides realign themselves to fit the amount of carbon in the sequence. The translated amino acids is a form of the nucleotide this clearly shows how the processes enable amino acids convert from one form to another depending on the strands.
References
Shortliffe, E. H., & Cimino, J. J. (2006). The Biomedical Informatics: Computer Applications in
Health Care and Bio-medicine (Health Informatics). New york: Springer.
