The In-situ mid-IR spectroscopy can be considered as one of the ideal methods of monitoring bioreactors. This is as the nutrients consumption for the amino acids is monitored together with the waste products evolution for the lactates. The application of fiber-optic probes is part of the appropriate ways of turning the methods into real-time tools for monitoring (Vogel & Todaro, 2006). On the other hand, it is difficult to fully engage monitoring methods among the reactors which require sterilization while the insertion of the non-sterile probes and other monitoring devices become rather impossible (Roy, 2011). The interchangeable detachable and Remspec ReactionView system head can be plunged into the sampling port prior the sterilization, for instance through autoclaving. This is to allow the sampling probes to be sterilized across with the fundamental reactor.
The fiber-optic cable linking the spectrometer to the sampling head externally is connected upon the complete set-up of the reaction for ensuring that there lacks an intrusion of the sterilized reactor’s interior. This means that the In-situ mid-IR spectroscopy opens up the chemical aspect in the fermenter (other reactor) for purposes of measuring waste products and nutrients including the dissolved carbon dioxide in real time and directly (McNeil & Harvey, 2008). The entire composition of the mid-IR spectrum is provided in a range of calibration methods as deemed applicable. In the above demonstration, peak-fitting models and simple peak heights graphed on the time are aimed at providing the appropriate outcomes. In a production or pilot-plant setting, and for the longer-term laboratory studies, the chemometric methods will have the highest likelihood of remaining as appropriate (Kale, Kale & Gokhale, 2008). PLS models can be involved for the species which are monitored and automatically operated against all spectrums while recording it to avail real-time trends.
References
Kale S. R., Kale R. R., Gokhale. S. B., (2008) Biotechnology and Fermentation Process. New York: Osprey Publishing
McNeil, B., Harvey, L., (2008) Practical Fermentation Technology. New York: John Wiley & Sons
Roy, M. J. (2011) Biotechnology Operations: Principles and Practices. New York: CRC Press
Vogel, H. C., Todaro, C. M. (2006) Fermentation and Biochemical Engineering Handbook, 2nd Ed.: Principles, Process Design and Equipment. New York: William Andrew
