Abstract analysis
Iyamu et al. (2014) has investigated proteins produced in HNSCC cell line SQ20B e cultivated in the serum free environment, later each protein has been cut into 10 pieces and analyzed using join liquid chromatography mass spectroscopy techniques, what enabled to show “prominent changes in the proteome when AHSG expression is diminished” (Iyamu, 2014).
Mass spectroscopy used in this research was developed in 20th century. It is selective and sensitive for the developed procedures. If the methodology is accurate the technique allows to determine even the slightest difference and thus to identify the molecules with nearly 100% accuracy. On some cases, it could be expected that the conditions chosen for measurements could help to distinguish similar molecules of different structure.
Still it lacks the number of developed procedures. The metabolisms of different substances and during different processes is quite different, so the MS result is affected by the chosen methodologies. The methodologies first were coming from the pharmacology, but still the interaction of the proteins and other produced chemicals can differ from the interaction induced by drugs of the similar nature, thus the real procedures should be adopted.
The preliminary results gave a good start for further identification of “mechanisms of tumorigenesis associated with AHSG”. After the mechanisms are finally distinguished, the research will be focused on “possible therapeutic targets for HNSCC”. (Iyamu, 2014).
Effects of Stimulatory and Inhibitory Neurotransmitters on Pancreatic Cancer Cells and Xenografts in Nude Mice. Hussein Al-Wadei
The research is focused generally on pancreatic ductal adenocarcinoma (PDAC) cancer processes. This particular study “has tested the hypothesis that social stress stimulates the growth of human PDAC xenografts via nAChR-mediated release of stress neurotransmitters and the associated activation of β-adrenergic signaling”. (Al-Wadei, n.d.)
Specific proteins were determined using western blotting techniques. It allows separation of the protein mix, thus determine different proteins considering their size, charge, and/or conformation. It is also possible t determine several proteins. Naturally the investigated materials possess several targets and their simultaneous identification could be more profitable and save the matter. Still this process is time-consuming what limits its to the fundamental researches. The need to optimize the experimental conditions is also not that favorable, as the research process can be less effective due to the inadequate conditions or the time spent for an optimization.
The poor therapeutic response now can be attributed not only to the nature of therapeutic agents used, but also to the social stress. This is very essential for the effective treatment of patients.
The future research could be focused on the investigation of effectiveness of the psychological stress reduction strategies together with therapeutic agents to improve the overall positive response to anticancer therapy.
Works cited
Iyamu et al., Shotgun proteomic analysis of human head and neck squamous cell carcinoma cell line SQ20B with diminished AHSG expression. BMC Bioinformatics, 2014, 15(Suppl 10), 35.
Al-Wadei H., Effects of Stimulatory and Inhibitory Neurotransmitters on Pancreatic Cancer Cells and
Xenografts in Nude Mice, n.d.
