Induction of Apoptotic Program in Cell-free Extracts: Requirement for dATP and Cytochrome c
This research sought to establish that cells that are through the process of apoptosis would have an increase the level of cytochrome c to their existing cytosol during vivo. This would infer that mitochondria of the cells would continue to work during apoptosis activity thereby initiating the production of cytochrome c The apoptotic program is brought about by the introduction of dATP. According to this research the cytosol of the cell produced approximately 15 kDa protein. This amount of protein meets the threshold that can initiate vitro apoptosis. Based on the protein patterns it was clear that the protein produced in this case was cytochrome c. When the produced cytochrome c in cytosol of the cell is removed, it was observed that the level of apoptotic activity significantly reduced. When cytochrome c was reintroduced to the initial extract the level of apoptotic activity increased.
Approaches and Experimental Designs: Are they appropriate and adequate to address the question?
This research employs quantitative research design. This allows the research to be value free. This is because the conclusions and discussions that are made on the research are based on collected natural data. The collected date is used as the inference point in making arguments and suggestions in the research. This is an appropriate research design to enhance the credibility of this research.
Main Claims: Significant? Have adequate proof or maybe a bit overselling?
The main claims in this research are adequate and not overselling. This is because the claims are not based on qualitative data but empirical research that are obtained from the experiment conducted. It is clear that the main claims in the research are based on the data collected.
Quality of the Data:
The experiments employs necessary controls to ensure the accuracy of the conclusions made in the research. For example, the experiment involves the depletion of cytochrome C from the initial cell extract to find out whether this would affect the level of the apoptotic activity. This control measure is conducted to make sure that cytochrome c from the cytosol of the cell has effect on the apoptotic activity.
Is the data interpretation accurate?
The interpretation of the data is accurate. This is because the research employs control measures in the experiment to make sure that the inferences made in the experiment are accurate. The research also uses charts and graphs to patterns in the experiment. This helps ascertain that the research methodology and design is accurate based on the results.
Are the conclusions supported by the data? If not, what other evidence is required?
The conclusions of this experiment are adequately supported by data. The research uses data to establish the level of apoptotic activity at different stages of the experiment. The use of natural data also allows the research to look for patterns with the experiment. The researchers are able to attain this by using graphs and charts. This graphs and charts illustrate the how the experimental activity progressed over time. One of the advantages of using data in this experiment is that the researchers can be able to identify any glitches that might have affected the accuracy of the experiment.
