Abstract
In the field of medicine, histopathology process is undertaken by pathologist.The aim of undertaking histopathology is to examine the existence or the possibility of a disease in the body tissue of a living thing. In addition, the process of histopathology is best practiced on the specimen of body tissue taken during the surgery of a patient. Here, the process involves a series of activities where surgery helps to remove body tissues for examination. After the surgery, the body tissue removed is placed in a fixative place where no external toxicities can contaminate the specimen. This forms the basis for preparation of viewing of the tissue using microscope to examine the disease. However, if the body tissue is large, a pathologist is mandated to take a sample of the tissue and subject it for histopathology. Further, the process of chemical fixation is subjected to the tissue to help remove unwanted chemicals such as alcohol. Water is also removed from the sample through the process of dehydration through the use of a chemical known as xylene. Afterwards, the tissue is cut into slices where a very small slice is used in the examination of the disease. Alternatively, a section of the tissue can be frozen. A device known as microtome is used to slice the section frozen into thin slices. Staining is done on the frozen thin slice. The disadvantage of frozen thin slice is that it requires time to process and it is costly in terms of the equipment needed. The importance of staining is to remove and reveal any presence of cell in the tissue. Finally, the slides are subjected to examination in a microscope by a pathologist. Where cancer was examined using the historical images, then, depending on the report, cancer will be diagnosed. Therefore, in histopathology, the interpretation of historical images after examination is crucial in helping diagnose specific disease. In particular, this paper focuses on the use of Programmable Supercontinuum Pulses. This technique helps in the process of applying multiphoton microscopy. As a result, it helps the technicians to obtain a histopathology which is free of stain.
(Below are the references that will be used to expand the research)
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