Pressure has over the time has been an important aspect in the thermal sterlialization of food . This is an alternative thermal process of food preservation which is highly effective(PATS) .The (PAT) is important in that it releases the diplicinic acid also known as the (DPA) from the individual endospores. It also rehydrates the core of the endospores and allows the process of the spore inactivation. A good combination can include 200 to 800 mpa which will also be at one hundred and twenty degrees, this combination can effectively eliminate any resistant spores of the organism Clostridium Botulinum. At a temperature above one hundred and twenty degrees Celsius pressure is likely to fall to accelerate the process of the thermal inactivation or it may even exerts a protective effect.
On an event whereby Antimicrobial are acting with PATS may also enhance the inactivation of the endospores thus ensuring the safety at a reduced treatment intensity .The chemicals element Nisin and Reutericylin also experience antimicrobial activity against the various endospores. Nisin is mainly a spore forming lantibiotic while reutericylin is mainly a protein ionosphere. Nisim is majorly responsible for the preforming spore inactivation. This usually has no effect or even any form of attenuated pressure induced which is required for the inactivation of the various inner bacterial endospores.
The endospores tend to have multiple distinct layers that often contribute to the resistance and the required processes of the metabolic dormancy. The process of the dehydration of the spores is a main contributor to the endospores main resistance and the related metabolic dormancy. This process of the dehydration of the spores is also a main contributor to the actual endospore resistance. Endospores also possess an outer membrane which is mainly responsible for the process of sporulation, the inner membrane also separates the dehydrated core from the hydrated exterior core .The lipids present in the membrane are usually compressed during the process of germination before being under the process of thermal inactivation. The disruption of the inner membrane of the individual endospores tend to rehydrate .This core allows inactivation of the endospores by the presence of the antimicrobials .
A new improved understanding that may guide to understand the process of the effect of pressure on the endospores membranes will have a lot of significance in improving the overall control of the endospores by pressure .It will also facilitate the main selection of the antimicrobials which are necessary to support the pressure assisted sterilization process .Little information is known on the overall effect of pressure and thermal application on the membranes of the endospores during the process of pressure processing on them.
This study strived to examine the overall effects of thermal process and pressure when combined with reutericyclin and nisin on the membrane and the fluidity of the individual endospores of the species Clostridium is also vital .This two chemicals Nisin and the reutericylin were selected mainly because they both possess membrane which are active but they differ majorly in their modes of action and the effects of their space survival after the application of heat and pressure on them .Therefore it is highly important to note on the importance of heat together with pressure on the major role they play in various process which affect the endospores inactivation .
The table below is a representation of the effect of heat on Endospores under different conditions and concentration. The endospores are subjected into various heat conditions which will therefore be producing varied results suitable for the experiment.
Previous data for action of heat on bacterial endospores
Pour plate colony count1/1000(10-3) dilution at different times in minutes
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