Assignment #4 (see Blackboard site for due date)
Peer evaluation (assign 12 points, total, based on the relative contributions you and your teammates made toward accomplishing the design goals for this assignment):
Teammate #1 (Name: ):
Teammate #2 (Name: ):
Teammate #3 (Name: ): if only 2 teammates, leave this blank
Summary of design process:
1. a. What is the lowest concentration of glucose that your sensor will accurately measure?
0.5 × 10–6 M
b. For this concentration, calculate the amount of enzyme on the electrode and the corresponding current that will generate when expose to the lowest glucose concentration. [Show final, neat version of the calculation(s) here. Show the full calculations with all iterations in your LogBook]
The linear regression equation was I (10–5 A) = –0.05279 C (10–7 M) – 3.8333 (n = 8, r = 0.995) with a detection limit of 6.26 × 10–8 M at 3σ. The current is proportional to the amount of glucose concentration and continues like that to 0.1mM
2. a. What is the highest concentration of glucose that your sensor will accurately measure?
12 × 10–6 M
b. For this concentration, calculate the amount of enzyme on the electrode and the corresponding current that will generate when expose to the highest glucose concentration. [Show final, neat version of the calculation(s) here. Show the full calculations with all iterations in your LogBook]
The linear regression equation was I (10–5 A) = –0.05279 C (10–7 M) – 3.8333 (n = 8, r = 0.995) with a detection limit of 6.26 × 10–8 M at 3σ. The current is proportional to the amount of glucose concentration and continues like that to 0.1mM
Other documentation required:
1) Scan/copy all pages from your logbook pertinent to this Assignment into a PDF file.
2) Convert this Worksheet into a PDF file
Combine these into one PDF file and upload to Assignment#4 on Blackboard
This paper presents a simple method through which a glucose biosensor can be prepared on the basis of glucose oxidase enzyme placed in a Nano composite ingredient made up of single-wall carbon nanotubes or ionic liquid.
On the basis of electrical conductivity, as well as, a wide window made up of ionic liquid and the electric property that the single-wall carbon nanotubes have, the glucose sensor is developed. The material used for building the biosensor is the IL-SWCNTs which is used for immobilizing glucose oxidase. The sensitivity is shown, response is there and stability too.
12 × 10–6 M, on the higher side. The detection limit, as indicated above, is the 6.26 × 10–8 M.
Experimental
The reagents were glucose oxidase, the ionic liquid, and the Nafion (5wt %). There is also 0.1M PBS which contains 0.1M KCl.
The electrochemical experiments are done on a CHI 832 electrochemical analyser, using a 3 electrode system which comprises of a modified GCE having a 4mm diameter as the working electrode, a platinum wire as the auxiliary electrode, and finally Ag/AgCl as the reference electrode. The potential on the electrodes for a few concentration values are noted and plotted on a graph against the currents on those electrodes.
Works Cited
Le, Lan-Anh, Hunter Jacob Ross and Victor R. Preedy. Nanotechnology and Nanomedicine in Diabetes. CRC Press, 2012. Print.
Pethig, Ronald and Stewart Smith. Introductory Bioelectronics: For Engineers and Physical Scientists. Chichester, West Sussex, U.K: John Wiley & Sons, 2013. Internet resource.
Schlesinger, Mordechay. Applications of Electrochemistry in Medicine. New York: Springer, 2013. Internet resource.
