Gel Reports Samples For Students

ABSTRACT:

A plasmid DNA is an extra-small circular structure commonly found in bacteria which is capable of replicating by itself in the cell. These are used in genetic engineering as vehicles to carry and make copies of a particular gene which can produce desirable effect. In this report, we discuss the isolation and purification of DNA fromEGFP-LC3 plasmidusing a modified alkaline lysis method and QIAprep spin columns. The concentration of the isolated EGFP-LC3 plasmid DNA was found to be 82.868µg/ml. The ratio of ODs taken at A260nm/A280nm was found to be 5.314 which could be because improper washing step which has ...

Introduction

The discovery of restriction enzymes (REs), bacterial transformation, and agarose gel electrophoresis caused the sudden increase of popularity of molecular biology in the 1970s. Restriction enzymes allow the manipulation of specific sites of an organism’s genes and are commonly used in many laboratories worldwide. Restriction enzymes cut DNA in many different ways and can produce sticky ends and blunt ends (Weaver, 2012). The most abundant type of restriction enzymes is Type II, which require magnesium cations as cofactor (Pingoud et al., 2005). Aside from their popularity is recombinant DNA technology as a “cut and paste” tool, estriction enzymes can ...

Energy gel case:

Overview
HPC is a company in the energy foods industry. Currently, the company has three arms, food, drinks, and international operations. The company sells energy drinks as well as the quick-pro energy bar. The head of business development Harry Wickler has come up with a comprehensive business plan for the introduction of energy bars in the market. However, there have been problems because not everyone agrees with his reasoning. In particular, Mark Leiter the head of the quick-pro energy bar product disagrees with Wickler’s cost calculation for the introduction of the new product as well as the cost ...

Aim: To separate Green Fluorescent Protein (GFP) using poly-acrylamide gel electrophoresis (PAGE).

Introduction: Bioluminescence observed in many marine organisms is due to a protein called as Green Fluorescent Protein (GFP). Since its discovery, GFP has been useful in biotechnology due to its versatility and ease of transformation in E. coli (Chalfie et al., 1994). GFP is a 26.87 kilodaltons (kD) protein composed of 239 amino acids. It has a barrel structure with a fluorescent chromophore embedded deep in the interior of the protein. The chromophores in GFP are excited at a wavelength of 395 nm, and when the electrons of chromophores drop to a lower energy state they emit fluorescent light of 509 nm (Ormo et al., ...

Abstract

A polymerase chain reaction also known as PCR refers to a technique that is used in the amplification of a DNA fragment making huge numbers of the original fragment. The process uses an enzyme known as DNA polymerase to multiply the DNA fragment. Other components that are necessary for a PCR reaction are the DNA template that is provided by the sample being multiplied, the primers, and nucleotides. The experiment aimed to examine a number of human DNA polymorphisms, the PV92 Alu polymorphism and the mitochondrial control region using human DNA. This was achieved by isolating the mitochondrial DNA from the mouth cells ...

Biology

Abstract
Paternity test is usually performed to individuals, where their DNA are compared to other people DNA to see if these people or person, is parent to the individual or are related. The method involves use of restriction enzymes to cut DNA from samples, which are then analyzed using gel electrophoresis. Gel electrophoresis will separate the DNA according to size and charge. They will be viewed on gel as bands. Normally, the DNA patterns are usually unique for an individual. Therefore, if there is a match is patterns from the analyzed samples, then there exists close relationship between two ...

*Corresponding author. email:

Abstract

Polymerase Chain Reaction is a useful technique for the detection of genes done through the amplification of specific sites. Alu elements are a polymorphic group of elements found in the primate genome amplified by retroposition. The Alu element help in the polymorphism of the populations. The aim of this study was to use the PCR technique to detect the Alu insertion TPA-25 in cells from student population and its relation with genotype frequency, allelic and ability to establish the Hardy-Weinberg equilibrium. Samples were taken from 81 students. Cells were obtained by mouthwash and Polymerase Chain reaction ...

Introduction

With the help of SFM or Sailfin molly fish as an emerging organism, we can have a better understanding of the theory of evolution. Some specifications such as the environmental impact, population biology and mating behavior can be revealed by studying the Sailfin molly fish. The “Guppy” genome is the closest relative of the sailfin molly since annotated. However the sailfin molly genome has to be annotated and not been done yet. In order to annotate sailfin molly genome we would need to create a proper polymorphic marker and some websites will help us to accomplish our goal.
Creation ...

Lab partners

The transformation of bacterial cells using Puc18 plasmid as a vector
Abstract
In molecular cloning experiments, the DNA to be cloned is obtained from an organism and then broken into smaller fragments through digestion with restriction enzymes. The fragments of restriction enzyme digestion are then ligated /combined with some vector DNA to produce recombinant DNA molecules. These recombinant DNA molecules are then introduced into a host organism. When the host organism replicates, the recombinant molecule is replicated together with it. With time, large populations of transformed cells or clones are generated expressing the genotype and phenotype of the inserted ...

Price

Before agreeing on pricing, Gillette Company put the following factors into considerations: it checks on the fixed and variable costs, competition from other institutions, the objectives of the company, the proposed positioning strategies and the target group and willingness of the customers to pay for the prices set.
The pricing strategies used by the Gillette Company include penetration pricing, skimming pricing, competition pricing, product line pricing, bundle pricing and cost up pricing. In penetration pricing, the Gillette Company places a price that is low so that it can increase its sales and the market share of all the products. ...

NIVEAManagement

MANAGEMENT
Executive Summary
NIVEA is the initial label and most significant line of Beiersdorf, a derma care organization that was started in Germany. Beiersdorf’s common aim is to become the leading care label in the product categories and market. The agency plans to attain such goals by utilizing NIVEA as a star line strategically. NIVEA's communication purpose is to reinforce its brand as the leading derma care line and to update its brand image (Keller, 2008, p. 6). Lately, the organization has declared Blue agenda the label compass, which concentrates on reinforcing the NIVEA line. Nevertheless, the firm ...

*Corresponding author. email:

Abstract

Alu elements are a group of mobile elements amplified in primate genome through retroposition. One of the Alu elements is the TPA-25. This element presents a high degree of dimorphism within human population, showing different alleles frequencies in different human group. Polymerase Chain Reaction (PCR) technique allow the amplification of part of the nucleic acid, including DNA being detecting by agarose gel. The objective of this study was to compare the genotypes of several individuals by using the Alu insertion detected by the Polymerase Chain Reaction. Cell samples from 82 students were taken. Samples proceed from ...

Abstract

The central dogma of biology is performed within this study. A vector of T7 RNA Polymerase gene was inserted into E.Coli where it could be transcribed into RNA and subsequently translated into protein. The protein was extracted and purified. A successful clone was produced by inserting the vector into the expressing cell line and it was observed after achieved an optical density at 600 nm (OD600) of 0.84. A collected 20.87 ug of double stranded RNA was found. Additionally, a linear DNA template OD260: 0.04 µg/ml was transcribed. After expressing it was found that a total protein concentration of ...

Introduction

The most widely used model when investigating the breast cancer is T47D cell lines. These cell lines are easily spread, easily manipulated genetically and when used in the experiment, it provides good results. They have been used to examine new therapies and the and the breast cancer pathobiology1.
Over many years HER2 has 25% - 30% of human breast cancers have been caused by gene amplification. The monoclonal HER2 and the EGFR are the two HER2-targeted therapies that are used for approval in the most clinics. During growth, HER2 is used to regulate the cell growth and assist ...

Introduction

Gene expression is the process through which the information that has been encorded in the gene is firstly converted into messenger RNA and then finally to a protein. The study of gene expression is critical especially to the biological student and more so to one who would like to study biology related course like microbiology in future. Therefore, it is important to note that we study gene expression in order to determine how the genes and the proteins which they encode functions in the intact organism. Zhang (2006),
Another reason why we study gene expression is in order to ...

TA
ABSTRACT – Genetic engineering now has straight forward ecofriendly strategies to produce transformation in plasmids. The transformation process allows engineers to introduce DNa with specific desired capabilities into a host plasmid. In this way plants can be created that are able to resist a particular enemy or stressor without having to use toxics like herbicides and insecticides. The bacteria E. Coli was used to determine whether it can be made resistant to ampicillin or not. AmpR was integrated into the host cells and the tracer, GFP was used to identify expressed cells. The laboratory demonstrated that transformation by plasmids so ...

Day 1 - Cell Culture: At First an LB/amp plate was labeled with initials, the date, the name of the bacterial strain to be used (BL21) and the plasmid (pDHFR). Then 10 uL of reconstituted BL21-pDHFR was pipetted onto an LB/amp plate using sterile tip.The bacteria was streaked into four quadrants of the plate and after this it was covered immediately to avoid contamination. The plate was placed upside down inside the incubator overnight at 37°C and once colonies have grown to ~1 mm in diameter, they may be stored in a sealed bag upside down at 4ºC ...

Introduction

Reduction reactions are very important in chemistry as they are effective processes for synthesizing new compounds from existing ones. In organic chemistry, a compound is said to be reduced if it acquires new hydrogen. There are diverse reagents for carrying our reduction reactions. Two of these are sodium borohydride (NaBH4) and lithium aluminum hydride (LiAlH4) - the latter is a more powerful reducing agent compared to the former.
In this experiment, NaBH4 was used to reduce p-Nitrobenzaldehyde to form p-Nitrobenzyl alcohol. NaBH4was used over LiAlH4 in this experiment, because LiAlH4 would also reduce the nitro group of the aldehyde.

Experimental:

...

Abstract

In this experiment, benzene was treated with hydrogen in the presence of Pt/SiO2 as an example that can be applied on industrial operations to show that the most recently modernized methods of neutron total scattering that cover a wide Q-range allow the use of appropriate catalytic chemistry that can be measured on time domain to probe directly on site the hydrogenation of benzene within the catalyst pore. Based on this method, it is possible to study the rates of reaction on length scales that can provide measurements equivalent to the size of an atom or a nanometre. Furthermore, the ...

Introduction

Initially there were beliefs that, MMPs are jointly able to degenerate all constituents making up the extracellular matrix (ECM) together with basement membrane that confines their operations to tissue reconstruction as well as the preservation. Nonetheless, the contemporary studies also signify that MMP-1 is responsible for controlling the production or else the activation of the chemokines. At this point, it is critical to understand that MMP-1 is among other MMPs.
Similarly, the knowledge holds it that MMP- 1 cleaves a peptide bond beforehand a residue comprising of a hydrophobic adjacent chain, for instance Met and Leu. The other sideways chains cleaved ...

Objective 2

Introduction 2
General background 3
Resistance mutants 4
Hemoglobin mutants 4
Sickle Cell Anemia and Genetics: Background Information 5
Connection of Sickle cell with Malaria 6
Method: 7
Introduction of Recombinant DNA technology in diagnosing disease: 8
Discussion (implementation of recombinant DNA technology to solve the issue) 9
Conclusions 10

References 11

Objective
This report targets to study a group of people that has developed resistance to malaria. It will focus on the use of recombinant DNA technology to study this cohort and gain insights into the nature of this observation. This ...

Introduction

Orthopedics refers to the branch of surgery devoted to the diagnosis and treatment of illness, injuries malformations and deformities of the musculoskeletal system (Goriainov et al. 2014). The system includes the muscles, ligaments, tendons, joints and bones. Orthopedic engineering leverages biomaterials in the form of implants such as sutures, joint replacements, bone plates and so forth. It also deals medical devices to replace damaged and defective parts of the body (Goriainov et al. 2014,"Material, Technology and Profit” 1988).
Biomaterial is termed as "Any substance (other than drug) or combination of substances, synthetic or natural in origin, which ...

Molecular Diagnostics

Genomic Evaluation of Risk of Atrial Fibrillation
and Alzheimer’s Disease

Session You Attended

Abstract
The aim to of this series of experiments is to examine a patient’s DNA for association to atrial fibrillation (AF) and Alzheimer’s disease. The single nucleotide polymorphism (SNP) rs2200733, associated with an increased chance of development of atrial fibrillation. Two SNPs: rs439358 and the rs7412, located within exon4 of the human apolipoprotein E (APOE) gene are associated with increased risk of developing Alzheimer’s disease. Isolation of the DNA from patient white blood cells, followed by PCR-RFLP and cycle sequencing are among the techniques ...

Dihydrofolatereductase (DHFR) is an important enzyme in the formation of DNA (Goodsell, 2002). DHFR controls the amount of tetrahydrofolate in the cells, which in turn is an important cofactor for purine and thymidylate synthesis and ultimately in the growth and proliferation of the cell (Davidson &Sittman, 1999). The enzyme is common to many species on earth. Human and bacterial DHFR have a relatively low rate of primary sequence agreement at 26%. However, they both retain a common general structure.The key role that the enzyme plays in biology as well as the variations that the enzyme displays makes it an important target ...

Lab report discussion and results

Introduction
The experiment is aimed at studying about the protein content in the cells and tissues of various organisms and the ways of regulating the growth of some tissues in the body of the animals like in our case we used the rabbit tissues in determining the feasibility of the experiment. The process begins with the ability to look at the process of obtaining the protein from the sample given and how to quantify the amount of protein in such cells.

Method:

Protein extraction
The process of protein extraction involves three major processes which must be followed in ...

Analysis of Culture Cell KU812F with Three Protocols

Abstract
Protein analysis for cancer cell research has been found to be extremely reliable. The culture cell KU812F (chronicle myeloid leukemia cell line) was used for this laboratory. Globin gene switching was observed by evaluating the gene expression in the cells. Gene expression and protein folding were provoked. Gene expression is the process of transcribing DNA to cDNA and then used qPCR to study gene expression. cDNA was translated into mRNA which in turn is translated into a protein. mRNA concentration was analyzed. The three protocols were Reverse Transcription Polymerase Chain Reaction (RT-PCR), Enzyme-Linked immune sorbent assay (ELISA), and ...

NITROGEN AND COPPER DOPED SOLAR LIGHT ACTIVE TIO2 PHOTOCATALYSTS FOR WATER DECONTAMINATION

Abstract:
A new category of photocatalytic coating that can degrade chemical as well as bacterial contaminants under visible sunlight wavelengths was generated by the deposition of a stable photocatalytic TiO2 film over the internal lumen of glass bottles by means of a sol–gel technique. Such a coating was produced either in doped or undoped form. This coating used copper and/ or nitrogen for producing visible light-active TiO2 films that were normalized or annealed at 600 ◦C and were characterized by Raman and X-ray photoelectron spectroscopy. The degradation of methylene blue was seen to accelerate with doped and undoped TiO2 films ...

Sand can be classified into coarse sand and fine sand, based on particle size. The former is usually greater than 4.75 mm (retained in a No. 4 sieve), while the latter is less than 4.75 mm (passing through the No. 4 sieve) (1).The properties, including shape and texture, size gradation, moisture content, specific gravity, reactivity, soundness, and bulk unit weight, of different types of sand,influence concrete strength (2). The shape of sand particlescauses variations in the surface to volume ratio, and a larger surface to volume ratio improves bond characteristics but decreases concrete workability. The surface texture of sand ...

1) Iodine test.

Iodine test distinguishes starch. It helps in distinguishing a polysaccharide from monosaccharide, disaccharides and other polysaccharides. The test is also called iodine staining. The iodine reacts with coiled polymer molecules and turn bluish black. Non-coiled starch does not react with iodine thereby no bluish color observed. A positive result is when there is the presence of the bluish black color.

Procedure

1. Put two drops of the solutions to be tested in three different test-tubes.
2. Add two drops of the IKI reagent to each of the tubes containing different sugar solutions.
3. Keep the sugar at room temperature ...