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ABSTRACT – Genetic engineering now has straight forward ecofriendly strategies to produce transformation in plasmids. The transformation process allows engineers to introduce DNa with specific desired capabilities into a host plasmid. In this way plants can be created that are able to resist a particular enemy or stressor without having to use toxics like herbicides and insecticides. The bacteria E. Coli was used to determine whether it can be made resistant to ampicillin or not. AmpR was integrated into the host cells and the tracer, GFP was used to identify expressed cells. The laboratory demonstrated that transformation by plasmids so ...
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Antepartum testing seeks to prevent fetal death, especially in high-risk mothers. Thorp and O’Neill (2013) stated that the evaluation identifies risks for an intrauterine injury that may lead to death with the aim of offering timely interventions. Apart from this primary role, could the assessment be of benefit in human genetic engineering?
Although some scientist opines that genetic engineering in humans has positive effects on humanity, there are many adverse effects. For instance, human genetics tend to mutate over time. As a result, the choice of the “perfect baby” may not be a viable idea on the long-term ...
IntroductionFood consumption is one of the major requirements for humans; people eat to live with at least majority of people been blessed with a meal each day, whereas others may afford three or more. People are independent of culture and customs, eating remains a key facet in different celebrations all over the world among and within friends and families. Moreover, people wish to have a nutritious and healthy meal but the problem is “The safety of the food people consume.”The improvement of livestock and plants for the production of food and the use of diverse conservation skills have been in application ...
ABSTRACT:
A plasmid DNA is an extra-small circular structure commonly found in bacteria which is capable of replicating by itself in the cell. These are used in genetic engineering as vehicles to carry and make copies of a particular gene which can produce desirable effect. In this report, we discuss the isolation and purification of DNA fromEGFP-LC3 plasmidusing a modified alkaline lysis method and QIAprep spin columns. The concentration of the isolated EGFP-LC3 plasmid DNA was found to be 82.868µg/ml. The ratio of ODs taken at A260nm/A280nm was found to be 5.314 which could be because improper washing step which has ...
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The transformation of bacterial cells using Puc18 plasmid as a vector
Abstract
In molecular cloning experiments, the DNA to be cloned is obtained from an organism and then broken into smaller fragments through digestion with restriction enzymes. The fragments of restriction enzyme digestion are then ligated /combined with some vector DNA to produce recombinant DNA molecules. These recombinant DNA molecules are then introduced into a host organism. When the host organism replicates, the recombinant molecule is replicated together with it. With time, large populations of transformed cells or clones are generated expressing the genotype and phenotype of the inserted ...