Polymerase Reports Samples For Students

Abstract

The central dogma of biology is performed within this study. A vector of T7 RNA Polymerase gene was inserted into E.Coli where it could be transcribed into RNA and subsequently translated into protein. The protein was extracted and purified. A successful clone was produced by inserting the vector into the expressing cell line and it was observed after achieved an optical density at 600 nm (OD600) of 0.84. A collected 20.87 ug of double stranded RNA was found. Additionally, a linear DNA template OD260: 0.04 µg/ml was transcribed. After expressing it was found that a total protein concentration of ...

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Abstract

Alu elements are a group of mobile elements amplified in primate genome through retroposition. One of the Alu elements is the TPA-25. This element presents a high degree of dimorphism within human population, showing different alleles frequencies in different human group. Polymerase Chain Reaction (PCR) technique allow the amplification of part of the nucleic acid, including DNA being detecting by agarose gel. The objective of this study was to compare the genotypes of several individuals by using the Alu insertion detected by the Polymerase Chain Reaction. Cell samples from 82 students were taken. Samples proceed from ...

Abstract

A polymerase chain reaction also known as PCR refers to a technique that is used in the amplification of a DNA fragment making huge numbers of the original fragment. The process uses an enzyme known as DNA polymerase to multiply the DNA fragment. Other components that are necessary for a PCR reaction are the DNA template that is provided by the sample being multiplied, the primers, and nucleotides. The experiment aimed to examine a number of human DNA polymorphisms, the PV92 Alu polymorphism and the mitochondrial control region using human DNA. This was achieved by isolating the mitochondrial DNA from the mouth cells ...

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Abstract

Polymerase Chain Reaction is a useful technique for the detection of genes done through the amplification of specific sites. Alu elements are a polymorphic group of elements found in the primate genome amplified by retroposition. The Alu element help in the polymorphism of the populations. The aim of this study was to use the PCR technique to detect the Alu insertion TPA-25 in cells from student population and its relation with genotype frequency, allelic and ability to establish the Hardy-Weinberg equilibrium. Samples were taken from 81 students. Cells were obtained by mouthwash and Polymerase Chain reaction ...

Introduction

Avian influenza also is known as bird flu and results from viruses acclimatized to some birds. People may be infected with avian influenza when they get into contact with the infected birds or infected fluids from the birds (Capua and Dennis 10). In this report, the discussion is about the evolution of avian influenza, characteristics of the virus, epidemiology of the virus, as well as the mode of transmission. Moreover, the paper talks about the gene expression of the virus, infection of the human by the virus, evolution of avian influenza's vaccines, and criticisms surrounding evolution of the virus.

Overall features of the avian flu virus

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Introduction

The most widely used model when investigating the breast cancer is T47D cell lines. These cell lines are easily spread, easily manipulated genetically and when used in the experiment, it provides good results. They have been used to examine new therapies and the and the breast cancer pathobiology1.
Over many years HER2 has 25% - 30% of human breast cancers have been caused by gene amplification. The monoclonal HER2 and the EGFR are the two HER2-targeted therapies that are used for approval in the most clinics. During growth, HER2 is used to regulate the cell growth and assist ...

Introduction

With the help of SFM or Sailfin molly fish as an emerging organism, we can have a better understanding of the theory of evolution. Some specifications such as the environmental impact, population biology and mating behavior can be revealed by studying the Sailfin molly fish. The “Guppy” genome is the closest relative of the sailfin molly since annotated. However the sailfin molly genome has to be annotated and not been done yet. In order to annotate sailfin molly genome we would need to create a proper polymorphic marker and some websites will help us to accomplish our goal.
Creation ...

Introduction

Gene expression is the process through which the information that has been encorded in the gene is firstly converted into messenger RNA and then finally to a protein. The study of gene expression is critical especially to the biological student and more so to one who would like to study biology related course like microbiology in future. Therefore, it is important to note that we study gene expression in order to determine how the genes and the proteins which they encode functions in the intact organism. Zhang (2006),
Another reason why we study gene expression is in order to ...

Summary: Examination of a biotechnology patent
Theoretically, thermal cycling refers to a process of temperature regulation scheme that is used to strengthen variety of materials or lengthen their durability. Thermal cycling apparatus and method refers to the equipment and technique used to carry out thermo cycling processes. Improved thermal cycling apparatus and relates to the modern advanced inventions of equipments and methods of carrying thermoregulation processes and minimizing related movement of fluids from two zones with two different temperatures to control chemical reactions.
Background of the invention
Temperature is one of the main factors that have been found ...