Stain Reports Samples For Students

UNKNOWN #16

SUMMER 2013
INTRODUCTION
Purpose
The purpose of the lab is to separate characterize and identify microbes from unknown mixed culture using the morphological and biochemical methods.

Theory

The process of identifying bacteria is a systematic and careful process which uses various techniques to narrow down the bacteria types which are present in an unknown culture of bacteria. This process produces advantages for many factors of research of microorganisms and assists in the proper treatment of patients (Aneja 10). Several tests were performed to give the fermentation abilities, availability of specific enzymes and specific biochemical reactions.

MATERIALS AND METHODS

The experiment started ...

Introduction

Bacillus Subtilis are the organisms that are aerobic, Gram Positive, spore-forming belonging to the Bacillus genus. The organisms are together with other species that are closely related play a crucial role in disease, food poisoning as well as food spoilage. Lack of methods that are standardized for the identification of the organisms whether coming from the food or environment has made the tests to be difficult. Use of morphological as well as physiological tests have offered the best ways to identify organisms in the laboratory although there are inconsistent and lack of reliance in these methods.
Identification of an ...

The process of identifying an organism involves the determination of where an isolate or an organism needs to be placed within a group of organisms that are already known. The process is usually done to conform to a given scheme of classification. Most of the microorganisms that may be found in the environment require to be identified aiming at identifying the different species that may be found in a given environment. The identification enables physicists and health workers to associated different sources with different organisms especially those that cause diseases. Organism classification may also help in the diagnosis of different disease by ...

THE MICROSCOPE

The fundamental objectives of this report are to learn how to:
- handle and care about the microscope
- Identify various parts of the microscope such as the eye piece, arm, coarse adjustment knob, fine adjustment knob, base, nose piece, objective lenses, stage condenser, and Iris diagram.
- Define various terminologies used while using the microscope such as the total magnification, resolving power, working distance, parfocal and real and virtual images.
- And finally to observe stained slides of bacteria or the fungi.

RESULTS

Concerning how to care about the microscope, the following should be taken ...

Component One

The Data Quality Objectives (DQO Process) is a planning process that has been developed by the United States Environmental Protection Agency. This particular planning process is used when environmental information or data is used to make a choice between two specific alternatives or to make a derivation of contamination estimate. The process is also used in the development of acceptance and performance criteria that find use in the clarification of study objectives, defining of appropriate data type and the specification of potential errors of decisions that are then used as the basis for the establishment of the quantity and the quality of ...

Introduction:

Transmission electron microscope (TEM) is the type of a microscope that uses a beam of electron rather than visible light to illuminate the specimen. Besides, while light microscope uses glass lens to focus light to the specimen, TEM uses electromagnetic lenses for focusing of the electron beam. TEM has a higher resolving power than the light microscope because the electron beam it uses for illumination has a much lower wavelength than visible light. During the operation of TEM, some electrons passing through the specimen get scattered and disappear from the beam. However, the unscattered electrons reach a fluorescence screen ...

Identification of mystery microbe based on its morphology and biochemical properties

The experiment was conducted to detect the species of a given mystery microbe. The microbe was subjected to a battery of tests in order to identify its physical and biochemical characteristics. Based on the tests, different possible microbe species were eliminated to finally arrive at the right conclusion. The possible microbes included Escherichia coli, Providencia alcalifaciens, Enterobacter aerogenes, Staphylococcus epidermidis, Klebsiella pneumonia, Micrococcus luteus, Serratia marcescens, Mycobacterium smegmatis, Sarcina aurantiaca, Pseudomonas aeruginosa and Bacillus licheniformis.
The first test conducted was to detect the Gram staining properties of the given microbe. This helped in identifying whether the given microbe had a thin ...

Purpose

Materials and Methods
Gram Stain: the test was done to differentiate Gram-positive from Gram-negative organisms. A drop of the unknown 300 was suspended on a slide and the culture spread to an oven thin film. The culture was air dried and fixed over a gentle flame. Crystal violet stain was added over the culture and allowed to stand for 60 seconds. The stain was poured off and rinsed gently. Iodine solution was added on the smear, allowed to stand for 60 seconds and the stain poured off. The slide was rinsed with running water, and a few drops of decolorizer solution ...

Part 1

1.
1. Eye piece 5.Arm
2. Objective lens 6.Coarse adjustment knob
3. Stage 7.fine adjustment
4. Base
2. What is the procedure (step by step) which you followed to do gram staining? (20 points)
1. Obtain a tissue sample for the gram stain.
2. Two or three drops of the sample were placed on the glass slide.
3. Use the electric slide warmer, heat the smear. Alternatively, this can be done by quickly passing it over a flame. Although should be very quick to avoid distortion.
4. Add crystal violet to the ...

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INTRODUCTION:

Pathologists or microbiologists play a very vital role in the management and diagnosis of any disease. They get immense help by performing certain series of tests which aids them reaching to a conclusion. One such test is called as gram staining which is used widely as the most primitive test for the identification of various bacteria
According to Beveridge, the gram stain essentially delineates bacteria into two broad categories. It does that by giving color to these bacteria and bacteria that attains purple or violet color are ...

Organism # 6

Molecular Biosciences 101
Section #
Purpose
Materials and Methods
Gram Staining: Three circles were marked at the bottom of a clean glass slide and they were labeled as gram(+), gram(-) and unknown respectively. The three labeled circles were then inoculated with microorganism 6 using an inoculating loop. The bacterial smears were then air dried and heat fixed over a gentle flame. The slide was then flooded with primary stain, crystal violet for a minute by placing it on a staining rack. Excess crystal violet was washed off with a gentle stream of water. Thereafter, gram iodine was added and ...

Differentiation of white blood cells by light microscopy

Most mammals have blood consisting of three types of blood cells, Red blood cells (Erythrocytes), White blood cells (Leukocytes) and Platelets (Thrombocyte). These are collectively termed as hematocyte, which simply means blood cell.
These cells constitute to around 40% to 45% of the blood volume. The remaining consist of Plasma, which is referred to the liquid part of blood. The total volume of blood can be determined by centrifugation or flow cytometry. The 45% of blood component (blood cells) is termed as hematocrit. Another important component of blood is hemoglobin, which gives blood its red color. It is ...

Weather: Clear, warm, temp at 86 F (outside) and 68 F (inside)

Time: Arrived on scene at 2058hrs

This was in response to an emergency phone call at 2055 hrs, made by a female who was hysterical but managed to give precise location of incident. On arrival at house, I found an early twenties white female lying on the living room couch and she seemed traumatized and confused. I quickly assume she is the caller and it was later confirmed that indeed she was Miss Jaen Daugh. She was assisted from the apartment by my colleagues and taken to the ...

Aim: To separate Green Fluorescent Protein (GFP) using poly-acrylamide gel electrophoresis (PAGE).

Introduction: Bioluminescence observed in many marine organisms is due to a protein called as Green Fluorescent Protein (GFP). Since its discovery, GFP has been useful in biotechnology due to its versatility and ease of transformation in E. coli (Chalfie et al., 1994). GFP is a 26.87 kilodaltons (kD) protein composed of 239 amino acids. It has a barrel structure with a fluorescent chromophore embedded deep in the interior of the protein. The chromophores in GFP are excited at a wavelength of 395 nm, and when the electrons of chromophores drop to a lower energy state they emit fluorescent light of 509 nm (Ormo et al., ...

Project Title

At about 2:30 in the morning of September 20, 2014, Detective James Reid called in for assistance in processing a possible murder case in No. 306, White Ridge Apartments, located in the corner of Strange Valley and Liberty Streets. The undersigned investigator arrived at the scene approximately 20 minutes later.
No. 306, White Ridge Apartments is a single bedroom apartment and its current occupant was identified by the building management as Chuck Matthis, a single, white male working as IT technician at the Silver-Toned Company. The light at the lobby to the apartment was busted and the main door of the ...

of HT22 Cells derived from Mouse Hippocampus

Sections I and II

Biology
Section I
1. Introduction
Fluorescent protein development research has gained increased knowledge about the range in the color palette available from the Aequorea Victoria jellyfish. The wavelength emissions in the blue to yellow range are now better understood. [1] Another area that has seen a great improvement in knowledge is the understanding of emissions of the monomeric fluorescent protein emissions in the orange to far-red spectral region. [1] The spectral region of fluorescent in the orange to far-red is derived appropriate reef coral species. [1] This area of research ...

A product strategy is the final vision of a product that helps determine the roadmap that the product will follow to achieve the eventual purposes set for that product. A product strategy enables an organisation to have a defined market as the focal point. Product strategy helps organisations define what they are selling, what value consumers derive from their products, how the products are priced and the distribution of that product to consumers (Susan & Nikolaos, 2000).
OMO is a detergent manufactured by Unilever Company. Unilever is employing its product strategy to ensure that it maintains its market share. In their product ...