Enzyme Research Papers Samples For Students

Introduction

Aspirin is one of the most commonly used drug in the world. The generic name is acetyl salicylic acid and the drug belongs to the group of non- steroidal anti-inflammatory medicines, which works by inhibiting the production of compounds in the body that cause pain, fever, inflammation, and blood clots (Koester, 1993). Aspirin is also a member of the salicylate family of compounds as it is synthesized from salicylic acid, which was discovered in Oxford (UK) by Edward stone in 1793 and first synthesized in the laboratory in 1897 by a German scientist, Felix Hoffmann of the Bayer company (Rinsema, 1999). ...

Abstract

Enzymes are the large biological molecules that play the role of metabolic processes responsible for maintaining the human life. They have the ability of high selectivity, as natural catalysts. They accelerate both the specifity and rate of metabolic processes, from food digestion and DNA synthesis. Most scientific enzymes are proteins in nature, even though some RNA molecules which are catalytic have been studied. The structures of enzymes normally adopt a three dimensional structure, and in order to assists in catalysis, it may employ the organic and inorganic forms. This experiment is meant to describe the properties and characteristics of enzymes ...

Assignment #4 (see Blackboard site for due date)

Peer evaluation (assign 12 points, total, based on the relative contributions you and your teammates made toward accomplishing the design goals for this assignment):

Teammate #1 (Name: ):
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Summary of design process:
1. a. What is the lowest concentration of glucose that your sensor will accurately measure?
0.5 × 10–6 M
b. For this concentration, calculate the amount of enzyme on the electrode and the corresponding current that will generate when expose to the lowest glucose concentration. [ ...

In the healthcare perspective, according to Gennaro et al. (2000), biochemical reactions are the chemical reactions involved in biochemical and cellular processes and particular actions of substances that affect these processes. Understanding biochemical reactions lead to the “understanding of the molecular bases of diseases”. The knowledge of receptor sites and metabolism (among other things) direct healthcare measures in efficient therapy management. Knowing normal cellular communication and metabolic pattern chemical reactions determine conditions that are abnormal in disease states. To Giese (2013), the scope of pharmaceutical reactions involve those of pharmaceutical biomolecules and biomolecule analogues and the chemical reactions involving ...

Information Research AssignmentBIOL1070 / BIOL2149 / BIOL2303

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Save the assignment as: 2013 BIOL Info_Res_assignment_your given name_family name.doc

All questions must be completed to pass the library component of the course.
Q.1
1.1 Explain your research topic in 100-200 words.
Noodles play a very crucial role in nutrition provision to the world population. They are very popular since they are easy to cook and are very nutritious. Dried, wet as well as instant noodles are used in large quantities, in China and South-East Asia. Noodles are made by mixing what flour and salt to make white noodles or with alkaline solution to make ...

Introduction

Enzyme activity is affected and influenced by several factors amongst which include temperature, pH level among others. High temperatures, for instance, denatures the enzymes hence inhibiting their activity, low temperature, on the other hand, inactivates the enzymes making them unable to digest the food. Enzymes are catalysts which mean that they mediate the digestion of food and their assimilation into the blood streams. They oversee the conversion of the substrate into products without being destroyed or changed in any way. (Cohen, pp134)
The enzymes have a primary and tertiary structure. The tertiary structures of an enzyme are important in ...

Abstract

Tay-Sachs disease (TSD) is a group of autosomal recessive lipid lysosomal storage disorders caused by mutations of the HEXA gene, which codes for the alpha subunit of the enzyme Hexosaminidase A, one of three enzymes responsible for the normal degradation of GM2 ganglioside, a substance found in high concentration in the plasma membrane of neuronal cells. The classic acute infantile form, discussed here, is a neurodegenerative disorder with onset at four to eight months of age in which acquired capabilities are lost and general neurologic deterioration occurs, with death by age two to four years. It occurs with highest frequency in the ...

Bioinformatics combines the subjects of biology, mathematics and computer science in to a single field of study. There are various tools available for the biomedical researchers so that they are able to find out the desired biochemical, genomic and medical information. Search programs, data base storage and software programs are some types of bioinformatics tools that help in the analysis of proteomic and genomic data. The web based tutorial of bioinformatics programs will be used in this study to analyze the most important enzyme of fertilization called phospholipase C-gamma. This tutorial will use the bioinformatics tools easily acquired from the ...

Telomerase also known as telomere terminal transferase is a type enzyme in all vertebrates that extends Die ribonucleic acid sequence by repeating TTAGGG to the Die ribonucleic acid terminal strands in the region known as the telomere. It is found in adult germ cells, fetal tissues, and tumor cells. Telomere is the region of repeated nucleotide, which is made up of non-coding Die ribonucleic acid material. The region prevents loss of vital Die ribonucleic acid from chromosome terminals. This results to only a hundred to two hundred nucleotides being lost each interval the chromosome is duplicates, thus no damage is caused ...

For over the years, there is a relative increase in cases of children with birth defects. Recent statistics with regards to the American babies suffering from birth defects reveals that for every 4 and a half minutes, there is a new case of baby born with a birth defect, which can also be summarized as 120,000 incidents of child birth defects every year, and it is expected that the number of child victims will rise for the succeeding years (Center for Disease Control and Prevention). Birth defects on newborn babies may affect almost any part of their body ...

INTRODUTION

Catechol-O-methyl transferase (COMT) is an enzyme that plays a significant role in the metabolism of dopamine (DA) and amounts to approximately 60% or more of DA breakdown in the frontal cortex . The gene expressing the COMT enzyme could affect cognition and functioning of the brain via its action on dopaminergic function. A polymorphic variation occurs in the coding area of the COMT gene (a Guanine to Adenine replacement), changing the amino acid from valine (Val) to methionine (Met) at the codon position 158 (Val158Met) and consequently influencing the thermal tolerance and stability of the enzyme. Allelic discrepancies in enzyme ...

(Author)

Use of Restriction Enzymes in Pharmaceuticals
Restriction enzyme, which is also referred to as restriction endonuclease, is a class of enzyme that is helpful in cutting DNA at restriction sites. Restriction sites are particular recognition nucleotide sequences on double stranded DNA having the length of 4-5 base pairs. Restriction enzymes are also known deoxyribonucleases (DNases). Presently, there are four different types of restriction enzymes:
- Type I enzymes that are “classical” in nature, e.g. EcoKI, EcoBI, and EcoR124;
- Type II enzymes that are “orthodox” in nature, e.g. EcoRI, HindIII, EcoRV;
- Type III enzymes ...

Disease pathophysiology

Definition of breast cancer
Breast cancer is a malignant growth of cells in the breast. This type of cancer arises in the milk-producing glands when cells begin to divide in an uncontrolled way, forming a tumor. The most common neoplasm in breast cancer is the adenocarcinoma of the cells covering the terminal lobular unit (American Cancer Society, 2016; Stephan, 2016).

Prevalence of breast cancer

Breast cancer is the most common invasive cancer to affect women in North America, with more than 200,000 new cases each year, and worldwide, counting up more than 1,200,000. It is the second cause of cancer ...

I. Introduction
Advances in recombinant DNA technology and bioinformatics-based data analysis have driven the explosion of structural biology in the last two decades (Kelly, Jess, and Price 119). This has resulted to the identification of at least 2000 proteins annually and a growing need in performing structural studies in these new proteins. The current trend involves the concern of analyzing protein structures close to its natural conditions (and other conditions) and of measuring their rates of structural changes, an essential factor in their biological functions. The technique of circular dichroism (CD) has gained an increasing popularity in addressing these ...

1.A. Penicillins are β-lactam antibiotics in structure. The remodeling process takes place in the bacterial cell walls due to the presence of a layer called peptidoglycan layer, which includes the breakdown of β-(1,4) linked N-acetylmuramic acid and N-acetylglucosamine and cross-linking peptide chains . Cross-linking peptide chains provide rigidity to the fluid cell wall. Transpeptidase, an enzyme used in the breaking of peptide chains also reforms the peptide bonds after the restructuring of the cell wall. The penicillin inhibits the enzyme transpeptidase, which prevents the peptide bonds from reforming. Thus, penicillin makes the cell walls weaker, which results ...

Von Gierke Disease

Introduction
Von Gierke disease or Glycogen Storage Disease Type 1 (GSD-1) belongs to a group of rare metabolic disorders with a reported incidence of 1 case per 100,000 births per year. The clinical condition results from deficiency of key enzymes in the glycolytic pathway resulting in an increase in intermediate metabolites and accumulation of glycogen in the affected tissues. It is a genetic disorder with a natural history of severe developmental abnormalities and long term complications. This paper provides an overview of GSD-1 based on studies published in peer reviewed journals with a focus on its genetic determinants, clinical picture, available therapeutic ...

1.0 Introduction
Down syndrome (DS) is a genetic condition caused by trisomy 21 (the presence of three copies of chromosome 21 rather than two). Although the actual cause of Down syndrome remains unknown, several risk factors increase the chances of a baby being born with the disorders. The primary risk factor, is the maternal, with about 80% of the cases of Down syndrome occurring in babies born by women above the age of 35. Other risk factors are genetic predisposition (indicated by siblings, another baby or parent with Down syndrome), immunologic problems, hormonal abnormalities, viral infection or x-rays. The risk ...

Gene replacement refers to a technique which utilizes homologous recombination (Carter & Shieh, 2010, p. 265), to make alterations by either adding a gene, deleting a gene or including point mutations. Gene replacement attempts to cure most of the genetic diseases at molecular level. A successful gene replacement prevents a protein from causing harm, giving new functions to proteins, and recovering or enhancing the normal function of a protein. This technique is commonly used to prevent, treat or improve animals. This is a permanent process that is very beneficial to animals. The process of animal gene replacement involves several processes ...

Investigating how light intensity will affect the rate of photosynthesis, with the help of DCPIP, in Spinach leaves.

The aim of this experiment is to find out how the rate of change of light intensity affect the photosynthesis in spinach leaves.
Successful farming of food crops is very essential for both the nutritional and financial health of a nation. Governments of almost every nation invest land and labour to crop farming, as much as is possible, keeping in mind the other needs of a country. But, however, due to many reasons the maximum crop yield possible is not always achieved.
For the production of a healthy crop certain conditions are necessary. The primary among these conditions are ...

Introduction

Over the years, research has been uncovering more and more rare diseases, especially genetic disorders. Hereditary Angioedema (HAE) is one of such rare disorders of the blood that causes a disfiguring swelling of a part of the body, particularly the face, larynx, extremities, abdomen, and genitalia. It may range from a simple painful swelling of an extremity to asphyxiation from laryngeal involvement. Diagnosis and prognosis of this condition depends on a heightened sense of awareness of the symptoms of the disease by the managing clinician and the availability of effective interventions.
HAE is known to occur in people with a deficiency of a particular ...

Impact of Triclosan on Health

Introduction
Antimicrobial or antibacterial agents are commonly used as ingredients in many of the household products. This trend has increased the concern of growing level of chemicals in the environment that ultimately bring negative impact on human as well as animal health. Triclosan is one among them. It is a synthetic, and an antibacterial agent having broad spectrum that has exploded in the consumer market in last few years. There has been tremendous debate on the safety, regulation of its use and effectiveness of triclosan (The European Consumer Organization, (2012).
Triclosan contains antibacterial properties along with antiviral and ...

For more than ten decades now, aspirin has been a preferable and effective pain killer. However, its effectiveness in prevention and management of heart disease was established in 1970s. Based on research findings, the American Heart Association accentuate that; baby aspirin can effectively manage heart disease, particularly, those who have survived the same and in effect prevent heart disease to those vulnerable to heart attack (Halvorsen et al. 325). In concurrence with the above notion, the Food and Drug Administration establishes that; only those with a history of heart disease and those vulnerable to the same are permitted to use the ...

Introduction

Biomass is a biological material obtained from a living organism or a recently living organism. The word biomass is derived from two Greek words; bio meaning life, and maza meaning mass (Demirbas, “Fuels from Biomass” 43). Biomass means non-fossil and bio-decomposable organic matter (originating from plants, animals and microorganisms). The products, byproducts, residues and waste from agricultural processing, all constitute biomass. Bio-waste simply means non-fossil and biodegradable waste that is composed of organic matter. Bio-wastes include food and kitchen wastes, garden and park wastes, and wastes from food processing plants. Bio-waste is therefore an element of biomass.
Non-fossil wastes that are ...

Introduction: Virology and Classification

HIV, a member of the class of viruses called retroviruses (their genetic material is ribonucleic acid, abbreviated RNA instead of Deoxyribonucleic acid, DNA, like most organisms), is the virus that causes AIDS (Acquired Immunodeficiency Syndrome). Known in full as human immunodeficiency syndrome, HIV exists as two major strains; HIV-1 and HIV-2 and is believed to have a simian source (Tang, 2009). Basically, HIV originated from simian immunodeficiency syndrome commonly found with chimpanzees and certain species of monkeys inhabiting Western Africa. The two strains of HIV, HIV-1 and HIV-2, were discovered in 1983 and 1986 respectively before being discovered in human blood ...

Section 2. Drug Metabolism

1. i. Referring to the primary literature, investigate the metabolism of paracetamol; Using a diagram as an aid, explain the metabolism of paracetamol noting the types of reaction (functionalisation, conjugation) and enzyme families (CYP, UGT, SULT) involved in these pathways.
There are two primary metabolic pathways for paracetamol within the liver – the first and most common is glucuronidation, wherein the paracetamol binds to the glucuronic acid enzyme. Sulfation, which accounts for most of the rest of these occasions, has the paracetamol combining with a sulphate to form a nontoxic conjugate (Hendrickson et al., 2006).
(Hendrickson e al., 2006)
ii. ...

(Author, Department, University,

Corresponding Address and email)

Osteosarcoma, also known as osteogenic sarcoma, is a bone tumor commonly found in the childhood and adolescence. It has been commonly reported in patients in the age range of 15 to 19 years. Incidence of the disease is almost similar for both males and females. This disease develops in bone-generating mesenchymal cells (Huether & McCance, 2013).

Causes of Osteosarcoma

Rapid local growth can result in osteosarcoma and can increase the chances of mutation. Ionizing radiation, even in low doses, can become the cause of rapid local growth. It has been reported that even therapeutic radiations for other forms of cancer ...

Introduction

The pathophysiology of atherosclerosis involves a series of complex events that can be likened to that of a chronic inflammatory process, which results in the development of an atherosclerotic plaque. The first step in the process involves injury to the endothelial cells of the artery that results in endothelial cell dysfunction. The activated endothelial cells attract vascular smooth muscle cells (VSMC) and leukocytes, which proliferate and accumulate in the walls of the artery (Seidman, Mitchell, & Stone, 2014). These cellular components lead to the formation of a large amount of connective tissue matrix. This is the ultimate end point in ...

Background

Early scientists and nutritionists believed that the most vital nutrient in living organisms, for building and maintaining the body was the protein. The role of proteins as enzymes came into existence several years later. This was around 1926 when experiments showed that enzymes were proteins. Insulin was the first protein to be sequenced. Hemoglobin was the first structure of proteins to be resolved. Myoglobin was then resolved later.

X-ray diffraction analysis (X-ray crystallography) led to the determination of the structure of the proteins (three-dimensional structure). The purification of proteins can be made possible from cellular compounds. Several methods have ...

COPD

COPD is a chronic disorder characterized by obstruction in the airflow, occurring over a prolonged period of time. The leading cause of the disease is cigarette smoking. The obstruction in the airway occurs due to damage to the parenchymal tissues and the damage to the airway passage. The symptoms may respond to the medicinal treatment but the damage to the airways cannot be reverted back to the normal.

COPD DIAGNOSIS

One single test may not be sufficient to diagnose the illness. It will require a series of tests to reach to the diagnosis. Following are the required tests according to the COPD guidelines:
...

Cystic fibrosis

Cystic fibrosis is a disease relating to the exocrine gland function. It involves multiple organ system as a whole, but there are some specific organ dysfunctions caused by this condition. These are the chronic respiratory infections, and pancreatic enzyme insufficiency. There are also some forms of complications that are usually associated with the untreated patients. Pulmonary issues or involvement is found in 90 percent of patients surviving the neonatal period. It has been noted in recent times that the major cause of death relating to the cystic fibrosis is the end-stage lung disease associated with it.
In the affected individuals, ...

Introduction

Most of the world’s chronic diseases are preventable by long term dietary behavior. Out of the ten major causes of deaths for Americans, diet is a contributing factor to at least five of them. These diseases are such as cardiovascular diseases, cancer and diabetes. A report by the Joint World Health Organization on diet made in 2003 revealed that, most of the world’s chronic diseases can be prevented by regular physical exercise and maintenance of a healthy weight. This finding is valid up to date. According to this report, a person’s genome determined their susceptibility to disease while external ...

Introduction

Depression is a disease affecting a large part of the population today. Though many people experience sadness from time to time, depression is a much more complex disease. It affects a person’s daily life and often results in physical pain. Unlike the occasional feelings of sadness that fade away, depression lasts longer (NIMH, 2014).
Biopsychology involves the scientific study of the relationship between behavior and biology (Green, 2013). In the study of their relationship, biology and behavior mostly interact in the brain and nervous system. It will also apply to the analysis of depression in relation to the biology; this study ...

Importance of Nutrition

Nutrition is important because it affects body development and systems at all the stages of an individual’s life. Diet affects mood alteration, mental development, physical well-being, and behavior. Nutrition affects physical health because the amount and quality of food eaten determine the availability of energy needed and the nutrients. The nutrients in the food are important in coordinating the activities of the nerves and the muscles. Nutrition affects mental health in that the brain requires certain nutrients found in food for proper functioning. Poor nutrition can influence the mind by causing depression, poor sleep, fatigue and poor concentration ( ...

Teratogens are genotoxins that can potentially disrupt the genetic makeup of developing cells, resulting in aberrations that can manifest disabling physical and mental abnormalities in a fetus. Teratogens can cross the placenta and reach the fetus, which quashes the popular belief that a fetus is shielded from external harmful influences. This indicates that the placenta is incapable of blocking teratogenic metabolites from reaching the fetus. Teratogenesis can be defined as birth defects or congenital malformations occurring in utero due to exposure to external factors. Such factors could be environmental, chemical or biological in nature. Factors such as the time of exposure, ...

FREDERICK BANTING

Biography
Fredrick Banting took birth near Alliston, Ontario, Canada on Nov 19, 1891. He completed his schooling from Alliston, and went on to pursue a medical degree from Divinity College and graduated to become a doctor in 1916. In his urge to serve the country during the First World War, he joined Royal Canadian army Medical Corps. In 1941, Banting died in a plane crash .

Contribution

Banting assumed that if the hormonal secretions of Islets of Langerhans (a part of the pancreas) were extractable, it could act as a controlling agent for diabetes.Along with his assistant Charles Best, Banting started his experiments on dogs ...

Introduction

Herpes Simplex virus type 2 (HSV-2) is a virus that belongs to the alpha herpes viridae subfamily and causes genital ulcer diseases. The transmission of HSV-2 occurs most often due to a sexual intercourse in humans, although it can disseminate through physical contact with the lesions and saliva from the patients. Medical professionals may contract the infection and lesions on their fingers, if they inadvertently contact the patient’s vaginal mucosa or mouth, without using appropriate gloves. The HSV-2 infection is known to be asymptomatic in 80 % of the cases . Genital herpes is a frequent infection acquired during pregnancy and about 5 % ...

Amyotrophic lateral sclerosis (ALS): A summary

Introduction
Amyotrophic lateral sclerosis is a rapidly progressing neurodegenerative disease. It is also known as Lou Gehrig’s disease. It is a disease that targets the neurons (nerve cells) that control the voluntary muscles. ALS belongs to a class of neurological diseases known as the motor neuron diseases (MND). Motor neuron diseases involve the steady deterioration and death of motor neurons. Motor neurons function as the communication links between the voluntary muscles of the body and the nervous systems. These neurons are located in the brain and the spinal cord. Transmission of information and messages occurs from the motor neurons of ...

Abstract

This research paper will discuss the significant contribution of DNA fingerprinting in the criminal justice system for being able to provide a reliable basis for conviction. DNA fingerprint analysis is referred as an accurate indicator to determine the guilt and innocence of an accused. The history, importance and various DNA fingerprinting techniques shall be discussed. In addition, DNA fingerprinting is considered as more superior than other forensic science methods since the technology has improved over time. DNA fingerprinting has allowed the test of smaller amounts of material, faster testing procedures and more conclusive results. Finger ridge analysis is science and ...

Polymerase Chain Reaction

Polymerase chain reaction known as PCR was developed by Kary Mullis in 1983. It is a Molecular Diagnostic technology used for amplification of DNA. A single copy of DNA can be amplified to higher magnitudes with a thousand copies of a single DNA sequence. PCR has now been developed as a Molecular Diagnostic tool for Cancer and mainly to detect the early onset of leukemia or lymphomas. It is widely used as a diagnostic tool in the field of microbiology and is also utilized in detection of infectious micro-organisms y detecting specific disease causing genes. In the year 1993, Mulis was awarded the Noble ...

The World Health Organization defines genetically modified foods (GMO) as “organisms in which the genetic material (DNA) has been altered in a way that does not occur naturally”. It involves technology often referred to as gene technology, modern biotechnology or genetic engineering. The process allows for selective transfer of genes from one organism to another. The transfer of genes can be done between organisms of the same species or of different species. Genetic engineering has been practiced for many years. We often hear of cases where a specific gene is selected from an animal or plant and introduced into a different organism ...

The advancement of technology has precipitated a revolution in scientific understanding over the last several decades. Advancements in research tools has led to new insights into every facet of scientific discovery within the realms of chemistry, physics, and biology and all the sub-disciplines forming their constitution. Increased discovery has brought with it opportunities for cross discipline cooperation which has resulted in the formation of new disciplines and has galvanized scientists to press the boundaries of research and application as has never been seen in human history. One such field testing those limits is genetics. Today’s geneticists have expanded beyond ...

Diagnostic Tests

This paper will describe diagnostic tests performed in respect of acute myocardial infarction and heart failure. The two diseases affect the cardiovascular system. The tests to be described are cardiac enzymes analysis and chest x-rays. The former test is recommended for patients with acute myocardial infarctions while chest x-rays are prescribed for heart failure patients.
Cardiac enzyme analysis is recommended as part of the diagnostic profile for acute myocardial infarction. It is recommended because injured cardiac cells release cardiac specific isoeznymes that can be detected in blood aftter they rapture following insults such as sustained hypoxia. Other than ...

Abstract

Alcohol is used by millions of people on a daily basis. The consequences of chronic alcohol abuse on the individual are many such as, memory deficits, neuropathy, heart, and liver damage. However, even minute amounts when drank during pregnancy can have effects on the mother in the long term as well as more dire consequences on the developing fetus that can lead to a lifetime of deficits. Alcohol is a teratogen that crosses the placenta. Doctors cannot determine the exact reason why certain infants of mothers that drink develop dysmorphic and neurobehavioral changes but the fact is, is that fetal alcohol syndrome ...

Globally, there sit eh concern about food security. This has been propagated by the current changes in climate and the environmental degradation which has been propagated by the global warming issues. The rainfall patterns have changed and the production patterns in different parts of the world have been negatively affected. As such, there has been the need to come up with ways of curbing the food insecurity and ensuring that the people are food secure. Scientists, agriculturalists and basically everyone else has taken a step towards ensuring that there is food security in the world. Various interventions have been suggested, ...

Smoke inhalation injuries in fire victims

The clinical manifestations of acute and chronic smoke inhalation injuries are due to two processes; one, the direct effects the inhaled substances have on the respiratory system that is, pulmonary injuries and the systemic effects induced by the toxic gases such as cyanide and carbon monoxide absorbed. Pulmonary injuries can further be categorized into those affecting the upper airway and those below the level of the glottis. Smoke inhalation triggers a systemic inflammatory response. Diagnosis of smoke inhalation injuries is based on the presenting symptoms and a battery of diagnostic tests majorly blood gas analysis.

Body

Injuries of the upper airway occur ...

Introduction:

Chromosomes refer to a structure that consist genetic material and formed by DNA and protein. Chromosomes are coiled form of DNA that consist genes, nucleotides and regulatory components. Shape of chromosomes varies depending upon the species. In Prokaryotes, chromosomes are circular in shape and have one cell whereas in Eukaryotes, chromosomes are linear in shape. Bacteria’s falls in prokaryotes category hence have circular chromosomes. Virus is an agent responsible for causing deceases. Viral chromosomes consist of nucleic acid and protein. Nucleic acid could be either DNA or RNA; both do not exist together in viral chromosomes. Viral chromosomes can ...