Introduction
Drosophila Melanogaster, also known as the fruit fly, is an insect used as a classical model system to determine the mechanisms of sensory systems, including hunger and diet selection (Amrein and Thorne, 2005; Schwarz et al., 2013). It feeds on the fungi of rooting fruit, and prefers foods that include sugars and amino acids (Thorne et al., 2014). Carbohydrates are a major source of food in adult Drosophila, similar to in humans (Amrein and Thorne, 2005).
Food ingestion is a behavior that all animals display. Feeding behavior in animals is a link between physiology and behavior (Itskov et al., 2014). In many insects, it includes the foraging and detection of food, the initiation of ingestion and the consumption of the meal (Edgecomb et al., 1994; Qi et al., 2015). Particularly, Drosophila feed on the food surface by extending their proboscis into contact with the food and drawing it in (Wong et al., 2009).
The central issue in foraging behavior is related to how organisms adjust their activities in response to the nature and distribution of potential resources. Changes in behavior due to modifications in resource availability may affect the success of the organisms, especially at the efficiency and reproductive levels (Prokopy and Roitberg, 1984). Particularly, in D. melanogaster the foraging activity is related with the foraging (for) gene, which have alleles related to the rover (forR)and sitter (forS)behavioral morphologies (Kent et al., 2009). These two morphologies respond in a different way to the present of nutrients.
The purpose of this investigation was to determine the foraging and feeding behavior of fruit flies using D. melanogaster apterous by using the question: “Do hungry fruit flies prefer a yeast solution to a sucrose solution?” The null hypothesis being tested was that there is no significant difference between observed and expected results for yeast and sucrose as feeding preference in D. melanogaster.
Results
Discussion
In nature, Drosophila feed from material and microbes that come from fermenting or rooting fruit. On the other hand, in the laboratory, they can be maintained on a combination of sugar, yeast and water, as a basal medium (Bass et al., 2007). Quantities of flies feeding with yeast solution were higher at the beginning (30 min) than at the end of the experiment 60 (min). The highest quantity of flies was found at 60 min, feeding with sucrose solution. Burke and Wadell (2012) affirmed that, in Drosophila, detecting sweetness indicates the presence of sugar and possible caloric content, and it is also associated to memory in this organism. This assumption can explain, in part, the preference of sucrose in this experiment, as it can be related to the sweetness of this solution. Previous reports demonstrate a preference of sucrose over yeast, when flies can select its food (Lee et al., 2008). Also, when larvae are depressed with respect to a nutrient, either sucrose or protein, they later selectively feed from a diet having the missing nutrient (Schwarz et al., 2013).
Flies quantities
Since significant difference (p<0.05) was found, the null hypothesis was rejected, meaning that there is a difference between the feeding tests. Feeding with sucrose was the best if we compare numbers presented in Table 2.
Although one would expect that the mix of both feeding sources, yeast and sucrose, would be the best one, results didn’t show a good performance in this test. Schwarz et al. (2013) reported a preference for both in Drosophila larvae. Based on these results, hungry fruit flies prefer sucrose solution over yeast solution having a significant difference (p<0.001) between feeding tests, which demonstrates some of the foraging behavior of Drosophila in finding food sources and preferences. Other studies, including molecular studies can aid in the knowledge of foraging behavior. Studying these mechanisms in Drosophila can help to further studies in this organism and in other organisms, such as humans, where homology of some genes has been found.
References
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