This study aimed at characterizing the regulation of oxygen-sensing pathway in human mesenchymal stem cells (hMSCs). Cells were extracted from umbilical cord blood (UCB), adult bone marrow, and pediatric bone marrow. Expression of HIF-1a and PHDs in the three cells were determined under hypoxia and normoxia. The following activities were performed while conducting the study: cell culture and osteoinduction, characterization of hMSCs using flow cytometric method, hypoxic exposure and Western blot analysis, Quantitative Real -Time RT-PCR (Q-PCR), Glucose and Lactate Assays, Transmission Electron Microscopy (TEM), Determination of pH of the culture media, and Statistical Analyses. The results showed ...
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