1. a. The pKa value of an amino acid is an indication of how strong or weak an amino acid is, based on the said acid’s dissociation constant. An amino acid that has a high pKa value is weaker than on that has a low pka value. Given that an acid’s dissociation constant is Ka, the pKa value is given by the formula: pKa = -log10Ka pH = pKa + log ([A-]/[HA]),It follows that for Cys12, 7.4= 7.2 + log([Cys-]/[HCys]) 7.4-7.2 = log([Cys-]/[HCys]) 0.2 = log([Cys-]/[HCys]) 100.2= Cys-/HCys Therefore, Cys-/HCys = 10-4Similarly, ...
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Abstract
In the following case-study, Joshua is a newborn infant presented by his parents Samuel and Rachel to the pediatric outpatient unit of Robertsbridge, Sussex for screening and medical advice for sporadic vomiting at the time of breast feeding and seizure-like episodes. Samuel and Racheal also report sweet odor in the urine and loss of weight. Blood tests demonstrated increased concentrations of leucine and isoleucine. Genetic testing on the skin biopsies reveal mutation at Exon 6, c.659C>T of the of BCKDHA gene with a protein level p. A220V and Joshua exhibits about 1.5 % lesser than normal deficiency in the ...
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