The experiment aimed to show that Blm10 protein elevates the turnover of proteasomal substrate through an active gating mechanism. This was to be done by showing that Blm10 enhances degradation that is proteasome-mediated in vitro using hypophosphorylated unstructured tau-441 as the substrate.
The objective was achieved using various stains such as BY4741, yMS63, yMS94, yMS122b, yMS131, yMS152, yMS159, and yMS476 among others. To start with, the proteasomal activity was assayed by determining gate opening in yeast CP/20S proteasome by the C terminal peptides. Phenotypic analysis was also done by growing the stains overnight in YPD and diluted to a density ...
