Essays on Electron Microscopy

Abstract

The study of tiny particles and their applications have emerged as an important area of research in recent years. In particular, magnetic nanoparticles have attracted considerable attention due to their unique properties that make the particles useful in numerous applications. Accordingly, the present review investigates the synthesis, characterization, and biomedical applications of magnetic nanoparticles. The introduction section examines recent advances in nanotechnology and explains the significance of magnetic nanoparticles in scientific research. The section on results and discussion presents the findings of various researchers with a focus on the magnetic properties, synthesis, characterization, and biomedical applications of magnetic nanoparticles. ...

Abstract

The field of Nanoscience has evolved due to the other advancements in the other fields of study such as the Nanotechnology. While the latter is a subset of the former, there is credible evidence that points to the fact that Nanotechnology has been developed thanks to various areas such as biology, material science, engineering physics, chemistry and much more. It is the foundation on which the Magnetic Nanoparticles have been embedded. MNP is nanoparticle that has the magnetic manipulation ability. Thus, this paper focuses on the study of the Magnetic Nanoparticles especially how they are formed or synthesized and ...

Introduction:

Nanobiotechnology serves as an imperative technique in the development of clean, nontoxic, and eco-friendly procedures for the synthesis and congregation of metal NPs having the intrinsic ability to reduce metals by specific metabolic pathways. Silver nanoparticles are particularly interesting nanoscale systems due to the ease with which they can be synthesised and modified chemically. Synthesis of silver nanoparticles has been widely researched due to their interesting size and shape-dependent properties [26]. These novel properties can be tailored by controlling their size, shape, composition, structure and crystallinity. Due to their size features and advantages over available anti-microbial properties, chemical imaging ...

Barbara McClintock was a one time student at Cornell University under the auspices of one C. B. Hutchison, who was lecturing in genetics during the fall in 1921. Genetics had not become a widely accepted course during the time, and students used to study it as a preamble to becoming plant breeders. Having been born in Hartford Connecticut in 1902, she earned her first degree in 1923, her Masters in 1925 and a Doctorate in Philosophy in 1927. Her steady rise as a geneticist was inspired by C. B. Hutchison. She studied the genetic characteristics of maize concentrating on ...

Scanning electron microscope uses a beam of electrons to form images that reveal the topography and composition of a body. Before being focused on the object, incident electrons have high amounts of kinetic energy. Incident electrons interact with the surface of the specimen, and this interaction is determined by the rate of the acceleration of the incident electrons. Energetic electrons are usually released from the surface of the sample when the incident electrons come into contact with it. The interaction results in the formation of scatter patterns that interpret into the shape, composition, size, and texture of the specimen. ...

1. Introduction to the Technique Magnification and high resolution imaging are very important in materials science, especially at present when nanotechnology materials are being created. Magnification and high resolution imaging can help in the characterization, identification, and confirmation of the existence of the desired material. Magnification and high resolution imaging become problems when the size of the assessed material becomes too small; such as, they are beyond the micrometer scale. Ordinary techniques in microscopy such as light microscopy where in a traditional light microscope is used could not suffice. Fortunately, in 1931, two scientists named Ernst Ruska and Mx ...

Analytical methods to evaluate seed dormancy in legumes

Abstract Legumes are known to have multiple health benefits. The seed coat in particular contains a number of bioactive compounds which possess antioxidant activity. Of great interest is the relationship between the chemical composition of the seed coat and its role in seed dormancy. Modern technological approaches are currently used to further identify these compounds and determine their role in seed development; these include NRIS FTIR, mass spectroscopy, electron microscopy and chromatography. The following review describes the constituents of the legume seed coat and their respective roles in dormancy and other stages of seed development. The review also highlights ...

Introduction

The microscope is derived from two Greek words, mikros and skopeo. Mikros means small while skopeo means “look at.” Thus, the two words form the word mikroskopeo that refers to looking or observing small objects. Since the beginning of scientific developments, there has been an interest to look at the smaller details of the world around us. Biologists have taken the lead in the quest to uncover such small details through their desire to examine cells, viruses, bacteria, and colloidal particles. Material scientists have wanted to observe the limitations and inhomogeneities in metals, ceramics, and crystals. Geologists have also ...

George Emil Palade was a highly esteemed and a well renowned cell biologist of Romanian origin. He was born in 1912, in Moldavia, Romania. In 1974, he won the Nobel Prize in Physiology and Medicine for his pioneering work in Molecular biology. He shares this award along with Albert Claude and Christian de Duve. He was referred to as the "father of modern biology" and "the most influential cell biologist ever" for his innovative contribution to the field of cell biology. While he was a medical student, he was invited by Albert Claude, the noble-winning biologist from the Rockfeller University who ...

Electron microscopes refer to those microscopes that make use of an electron beam to light a specimen producing a magnified image. These microscopes are used to study structures that are either invisible through naked eyes or those that are too small to be clearly seen using a light microscope. The two major types of electron microscope are Transmission Electron Microscopy or TEM and Scanning Electron Microscopy or SEM (Mooney, 1996). Transmission Electron Microscopy employs a high voltage electron beam in order to create an image (NE, 2013). The TEM is made up of various components including electromagnetic lens system, electron source, sample ...

Abstract. The aim of the study was to validate the existence of vesicles in gram positive bacteria, bacillus subtilis. The visualization of the vesicles was carried out by various microscopy techniques. The measurement of vesicle size was performed and the amount of vesicles compared between various strains. The laboratory cultured strains was more effective in the study as they exhibited hyper production of the vesicles. The production of vesicles was also validated in environmental strain, however in much smaller amount. The vesicles were also visualized and isolated from biofilms that act as the natural environment of the bacteria. Proteomic ...

Biology

The electron microscope has proven to be invaluable in the study of proteins and has enhanced our understanding of cellular biology. The importance to understanding disease and trauma to the body as well as treatments cannot be overstated. Genetic research such as studies of the mechanisms of protein folding is better understood due to electron microscopy. The image of a sample such as a slice of a fibril or a “newly synthesized chain of amino acids” (Dobson, 2003, p. 884) created by an electronic beam. The Transmitting Electron Microscope (TEM) transmits the electron beam through a sample whereas the SEM scans the surface ...

- Toxicology With the help of an electron microscope, it becomes possible to carry out a detailed study of the toxic lesions in order to understand the toxicological events. - Electron Tomography and Sub-cellular Structure Electron tomography is a technique which is used to visualize and investigate the three-dimensional structural information about the sub-cellular macromolecular specimens. It is a three-dimensional electron microscopy technique used for sub-cellular imaging.

Principles of Function

- Electrostatic Interactions In an electron microscope, electrostatic and electromagnetic lenses are used to selectively control and focus the electron beam to generate images. In atoms, with the increase in atomic ...

Abstract

Microscopy is the science of viewing objects that are very minute and can only be viewed by an instrument called a microscope. The science began in the 16th century and has been progressing into viewing specimens at higher resolutions, greater magnifications, and in 3-D. These advancements are in the three sub-branches of microscopy which are scanning-probe, optical, and electron microscopy. The paper analyses the latest advancements in the three fields indicating what the invented microscopes can be used in science.

Introduction

In Biology, Microscopy is the technique of using microscopes to view objects that are very minute and can only be ...