Question 1
a) i) If genomic DNA from the wild type bacterium were digested with EcoRI, three different fragments would be detected. The approximate sizes of the fragments would be 1.625 Kb, 1.375 Kb and 0.25 Kb. This is because there are two different digestion sites for EcoRI within the region complementary to the probe, and therefore three different fragments would partially hybridize with it after the digestion. ii) There are only two restriction sites for BamHI shown in the diagram, but neither of them is within the region complementary to the probe, and both of them are upstream of this ...