Essays on Ligases

Vid30 complex participation in the degradation of CDC25 in yeast and their role in Ras/ cAMP/ PKA pathway

Background: Unicellular organisms such as Saccharomyces cerevisiae in its total life cycle is exposed to diverse and changing nutrient conditions. Thus, in order to survive and achieve a competitive advantage, it has adapted itself such that it not only uses the wide range of available nutrient sources but also the sources that are nutrient rich. It mainly uses fructose and glucose by rapidly fermenting to alcohol. In response to these nutrient conditions, the intracellular trafficking transcription and turnover of protein from nutrient transporters (e.g. Hxts) get regulated. Therefore, when the availability of the glucose increases, the expression of low- ...

Organization:

The term biotechnology refers to any process that involves biology and technology. Though the term was coined as far back as 1919 by Karl Ereky (Verma et al., 2011), biotechnology was established as a field of applied science only in the late twentieth century with the advent of recombinant DNA technology. Recombinant DNA technology is the generation and application of recombinant DNA molecules or rDNA. In a nutshell, recombinant DNA technology involved the cutting of a fragment of DNA and its insertion into a specific circular DNA molecule called a vector. The DNA is cut by enzymes called restriction endonucleases, and joined to a vector ...