Day 1 - Cell Culture: At First an LB/amp plate was labeled with initials, the date, the name of the bacterial strain to be used (BL21) and the plasmid (pDHFR). Then 10 uL of reconstituted BL21-pDHFR was pipetted onto an LB/amp plate using sterile tip.The bacteria was streaked into four quadrants of the plate and after this it was covered immediately to avoid contamination. The plate was placed upside down inside the incubator overnight at 37°C and once colonies have grown to ~1 mm in diameter, they may be stored in a sealed bag upside down at 4ºC ...
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