Background
Early scientists and nutritionists believed that the most vital nutrient in living organisms, for building and maintaining the body was the protein. The role of proteins as enzymes came into existence several years later. This was around 1926 when experiments showed that enzymes were proteins. Insulin was the first protein to be sequenced. Hemoglobin was the first structure of proteins to be resolved. Myoglobin was then resolved later.
X-ray diffraction analysis (X-ray crystallography) led to the determination of the structure of the proteins (three-dimensional structure). The purification of proteins can be made possible from cellular compounds. Several methods have ...