Essays on Phenol Red

Identification of the Unknown Microorganisms

Unknown #1 Unknown #2 1. The identification of bacterial strains is a common practice in clinical and medical fields. The ubiquitously present microorganisms can be both, beneficial or harmful to the host they attack or inhabit. Since times, several biochemical characteristics have been identified for many bacteria that commonly are related to humans. These characteristics and morphology have been arranged in a systematic way to identify the targeted unknown microorganism along with ruling out the ones giving negative results for the characteristic tests. This way the search was narrowed down for each of the two unknown microorganisms. The Gram ...

The process of identifying an organism involves the determination of where an isolate or an organism needs to be placed within a group of organisms that are already known. The process is usually done to conform to a given scheme of classification. Most of the microorganisms that may be found in the environment require to be identified aiming at identifying the different species that may be found in a given environment. The identification enables physicists and health workers to associated different sources with different organisms especially those that cause diseases. Organism classification may also help in the diagnosis of different disease by ...

Purpose

Materials and Methods Gram Stain: the test was done to differentiate Gram-positive from Gram-negative organisms. A drop of the unknown 300 was suspended on a slide and the culture spread to an oven thin film. The culture was air dried and fixed over a gentle flame. Crystal violet stain was added over the culture and allowed to stand for 60 seconds. The stain was poured off and rinsed gently. Iodine solution was added on the smear, allowed to stand for 60 seconds and the stain poured off. The slide was rinsed with running water, and a few drops of decolorizer solution ...

Introduction

Bacillus Subtilis are the organisms that are aerobic, Gram Positive, spore-forming belonging to the Bacillus genus. The organisms are together with other species that are closely related play a crucial role in disease, food poisoning as well as food spoilage. Lack of methods that are standardized for the identification of the organisms whether coming from the food or environment has made the tests to be difficult. Use of morphological as well as physiological tests have offered the best ways to identify organisms in the laboratory although there are inconsistent and lack of reliance in these methods. Identification of an ...