Click Chemistry describes the biocompatible reactions that are high yielding, that are very simple to perform, have wide scope, that need no chromatography to remove their byproducts and that can be conducted in easily removable solvents. The synthesis of first triazole was reported by Arthur Michael in 1893. In the 20th century, Huisgen discovered the catalyst of copper, 1,3-dipolar cycloaddition which required the temperature of 100 degree Celsius. This reaction of copper is a highly versatile reaction which does not require any specific reaction conditions and can easily be performed with various solvents, wide temperature range and pH, ...
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The transformation of bacterial cells using Puc18 plasmid as a vector Abstract In molecular cloning experiments, the DNA to be cloned is obtained from an organism and then broken into smaller fragments through digestion with restriction enzymes. The fragments of restriction enzyme digestion are then ligated /combined with some vector DNA to produce recombinant DNA molecules. These recombinant DNA molecules are then introduced into a host organism. When the host organism replicates, the recombinant molecule is replicated together with it. With time, large populations of transformed cells or clones are generated expressing the genotype and phenotype of the inserted ...
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