Lab Report: Ethanol Determination Experiment
Objective: “To Determine the Ethanol content in several beverages using the enzyme alcohol dehydrogenase.”
The Chemistry behind this experiment is simple enough to understand. In the reaction carried out in the experiment, Nicotinamide Adenine Dinucleotide (NADH) is formed which can be analyzed quantitatively to find the concentration of Ethanol. The reaction involves oxidation of Ethanol to Acetaldehyde. This oxidation takes place with concomitant reduction of NAD+ to NADH. This formation of NADH can be seen in the Δ absorption at 340 nm micro level. At this wavelength, the reduced co enzyme absorbs at a very fast rate than the oxidized one, ...
