Introduction
Steroids play a central role in enhancing human growth, development and normal health, since they take part in development of secondary sexual traits, reproduction, gene expression, neurological and cardiovascular functions and maturation among others. Accordingly, imbalances in the metabolic mechanisms of steroids in humans is associated with an array of health complications such as cardiovascular and neurodegenerative diseases and cancer among others. The foregoing underscores the importance of steroid profiling to establish its levels in humans, particularly in disease diagnosis, because it enables health professionals to understand a variety of steroid-related health conditions. For efficiency and accuracy, a good profiling method is ...