Thin Layer Chromatography of Amino Acids
Introduction The technique used for this experiment was thin layer chromatography (TLC). TLC is used to separate components of a mixture (Clark, 2016). The purpose of this experiment was to identify an unknown amino acid. The components will have different affinities to the stationary and mobile phases. The various components will travel at different rates based on their affinity to either the mobile or stationary phase (Clark). A polar stationary phase is used in TLC experiments and the mobile phase can be a combination of solvents or just one solvent (psu.edu, n.d.). TLC can also be used to monitor ...
