Essays on Transcription

Great Law of Peace

The one aspect that stands out is the use of the great tree to symbolize strength and peace. Its roots spread out in four directions like a compass. People who wanted to maintain peace in the Five Nations were required to trace the roots of the tree to come together and enjoy the shelter of the big tree (Wampum par. 4).

Declaration of Independence

The article outlines the principles of the U.S. Government as well as the constituents of the American identity one of the being mentioned at the beginning of the text that all men are equal and deserve happiness, liberty, ...

2) click /klɪk/; clique /kliːk/ candid /ˈkændɪd/; candied /ˈkændɪd/ tired /tʌɪəd/; tirade /tʌɪˈreɪd/ sweat /swɛt/; sweet /swiːt/ bade /beɪd/ or /bæd/; bad /bæd/ 3) (a) /kærәt/ carrot carat (b) /fraɪәr/ fryer friar (c) /kɔrs/ course coarse (d) /deɪz/ days daze (e) /ɝn/ urn earn (f) /noʊz/ nose knows (g) /ɡroʊn/ grown groan (h) /θru/ through threw (i) /hɑstәl/ hostel hustle (j) /ɡreɪt/ great grate (k) /taɪm/ time thyme ( ...

Qualitative Research

Brief Description The interview was conducted among two people on the topic “Interpretation of the migration crisis”, which turned to be quite challenging due to the sensitiveness of the topic. First of all, it was problematic to find participants, as the majority thought that the answers may affect their social or professional life, even though I made it clear that the participation is anonymous. Secondly, one of the respondents refused to continue answering the questions in the middle of the interview without explaining the reasons. However, as this option was discussed before the interview, it did not affect the ...

A proposal in partial fulfillment of the requirements for the degree of

Introduction Wheat (Triticum sp.), an extensively adapted crop, is one of the primitive and primary staple food crops in the world. Wheat is cultivated from temperate; dry to irrigated or rain-fed areas, to warm, humid or cold conditions. Such a high adaptability is attributed to its intricate genome (Acevedo et al., 2002). Wheat cultivation covers more land area compared to other commercial crops and endures to be an essential food grain for humans (Curtis, 2002). Estimations reveal that by 2050, the population of the world will reach 9.1 billion (34 percent greater than today); correspondingly, the food requirement and ...

Introduction

The large production of bisphenol A (BPA) in the world has been documented because of the increased usage of the same in various industries. The most critical industries that have been using this BPA include those manufacturing containers for foods and beverages, cigarette filters, thermal receipts as well as paper currency. With such a huge provision, it is critical that the basis of such developments only emanates from making the right choices over its uses because its production to a total of 2.7 million tons could be disastrous to the body. The main reasoning here is that the chemical ...

Transcriptional regulation of gene expression

Gene expression in eukaryotic cells is regulated at transcriptional, post transcriptional and translational levels (Tomizawa,  Lekstrom-Himes, and Xanthopoulos 17). Transcriptional control is the primary means of regulating gene expression. Eukaryotic transcriptional control functions at three levels: modulation of the activities of activators and repressors; variations in chromatin structure; and effect of activators and repressors on assembly of initiation complexes (Lodish et al. 368). Regulation of transcription initiation is the most widespread form of gene control in eukaryotes. However, in some cases, transcription may be attenuated and regulated at subsequent steps, such as during or after the transcription process (Cooper ...

Vid30 complex participation in the degradation of CDC25 in yeast and their role in Ras/ cAMP/ PKA pathway

Background: Unicellular organisms such as Saccharomyces cerevisiae in its total life cycle is exposed to diverse and changing nutrient conditions. Thus, in order to survive and achieve a competitive advantage, it has adapted itself such that it not only uses the wide range of available nutrient sources but also the sources that are nutrient rich. It mainly uses fructose and glucose by rapidly fermenting to alcohol. In response to these nutrient conditions, the intracellular trafficking transcription and turnover of protein from nutrient transporters (e.g. Hxts) get regulated. Therefore, when the availability of the glucose increases, the expression of low- ...

Introduction

The treatment for multitudes of diseases and disorders resulting from genetic reasons have been ambiguous since long as the only cure could be the correction of genes and DNA in the specific region. Gene therapy gave a promising horizon to the correction of such genetic defects whereby molecular tools are applied to reverse the mutation or any other changes in the DNA. The advancing biomedical research have offered several tools for gene therapy since last few decades that have been able to perform the desired roles but with one or the other limitations. Lately, CRISPR-Cas9 based tools for gene ...

Assignment 2

PART A - (Total 15 marks)

Cephalexin and Gentamicin The mechanism of action of amoxicillin is focused on the prevention of the synthesis of the bacteria’s cell wall through the binding and inhibiting of the cell wall transpeptidases (Katzung, Masters, and Trevor, 2012). Specifically, it attaches to the penicillin-binding protein 1A found in the cell wall of bacteria. Then, the agent disintegrates the lactam ring to allow the acylation of the penicillin-sensitive transpeptidase C-terminal domain. The formation of the cross-link of the linear peptidoglycan strands is prevented by the inactivation of transpeptidases. Since the third and last stage ...

In their informative poster, University of Dayton’s Biology Department researchers Ankita Sarkar, Janan Bati and Armit Singh seek to explain the complex genetic hierarchy of eye development through experiments which analyze the development of the eye of a Drosophila Fruit eye from birth to adulthood. According to the experiments, significant changes occur in the eye at the beginning of the third instar stage of development. It is at the third instar stage where the fly’s retinal gene differentiation occurs. According to the researchers, “a wave of differentiation sweeps from the posterior to the anterior margin” of the ...

Biology

(Department)

DNA and its importance in our lives

Introduction Deoxyribonucleic acid (DNA) is a long linear polymer located in the nucleus of a cell. It is developed from nucleotides and shaped like a double helix. It is related to the transmission of hereditary information from one generation to the second generation. DNA contains the information for the development as well as reproduction of an organism, thereby leading to a normal life. In the year 1953, James Watson, a biologist, and Francis Crick, a physicist, discovered the structure of DNA in a laboratory in England (LEROY, 2003). The structure of DNA helped scientists to know the process ...

Introduction

It is customary for the non-native speakers to substitute the correct pronunciation of the words with their versions of vowels and consonants. There are patterns of pronunciation of words in every language and it is believed that the non-native speakers utilize their habitual pronunciation of the new language. These patterns in language may persist at different levels. Some of the non-native speakers tend to learn new language faster, the others retain the traces of native language in their patterns of pronunciation through the years. This may root in different factors, such as the duration of practice in new language, ...

Abstract

Medication errors are common medical errors in healthcare settings that often lead to patient harm. Medication errors transpire during ordering, administration, and transcription of medications. Healthcare information technology is a promising strategy for prevention of these errors. In this paper, an article that reports on the application of technology to prevent medication errors was analyzed. The article is entitled “Effect of bar-code technology on the safety of medication administration”. It is authored by Poon et al. (2010) and reports on the methods and findings of a study that examined the impact of the use of eMAR technology on rates ...

Center for Domestic Preparedness (CDP) is one of the FEMA entities. It is situated in Anniston, Alabama. It provides training facilities for Weapons of Mass Destruction (WMD) under its federally controlled training center by the United States Department of Homeland Security (DHS) <https://training.fema.gov/>. The CDP is dedicated to the cause of preparing and enabling residents to respond to all risky occurrences. The primary job of CDP is to shortlist, educate, evaluate, and provide practical training to responders for handling emergency situations. Responders can belong to local, state or tribal communities <https://cdp.dhs.gov/about/>. The number of emergency responders has ...

Part B

The tests are designed to assess progression through the levels, and at any one level the prior language knowledge and attainment required is that defined in the assessment units in the ESL level below measure at the comprehension or higher levels of Bloom’s (Bloom, et al) Taxonomy.

The tests are designed for learners who are aged 18 and over, whose first language is not English. It is expected that the learners’ educational and employment backgrounds will be diverse, as well as their aspirations, literacy levels and language learning skills. This diversity is reflected in a range of material ...

Bio Exam

Codominant traits exist when two different alleles for a genetic trait such as flower colour, and hair texture are both expressed. The expression of codominance infers a relationship between two types of a gene. In codominance, the phenotypes of both alleles inherited from the parents are expressed ("Codominance - Glossary Entry", 2016).

Genetic expression feather colors in purple penguin ("Basic Principles of Genetics: Probability of Inheritance", 2016).

With the order of dominance as pd>pm>pl>pvl a 1 dark: 1 medium ration of phenotype among the baby penguins is expected. Marfan Syndrome is a genetic disorder that exhibits variation among affected individuals. The range of phenotypic severities is an example of variable expressivity. ...

Introduction

The total number of patients with cancer keeps soaring globally with around 14 million cases diagnosed by 2014. Each year, about 8 million people die of cancer-related illnesses globally. There are quite some remedies that have been proposed to mitigate in the cancer menace. This study is focused on cancer treatment by curcumin through tyrosine kinase inhibition. Curcumin is the primary coloring agent found in turmeric, a tuber of the herb Curcuma longa Lin. It has widespread use as a flavoring agent (Aggarwal and Bokyung 87). It is also well known for its medicinal value commonly used to suppress ...

Gene regulation involves the cellular process that aims to prevent and control the manner in which gene expression occurs. Gene regulation includes a range of mechanisms used by the cells to decrease or increase the production of specific products of the genes. Many sophisticated programs of gene expression are observed in biology that includes response to the environmental stimuli and triggering of other developmental pathways. Steps involving expression of genes can be controlled from the initiation of transcription, to processing the DNA, and to the mechanisms involved in the post translational modification of the proteins. Gene regulation is important for ...

Choose a gene that has been implicated in a human developmental disorder

FOXP2 is a gene that codes for a member of the transcription factors’ forkhead /winged-helix family, FOX. It is situated from base pair 114,086,309 to base pair 114,693,771 on the chromosome 7’s long (q) arm at position 31. The gene has 42 distinct introns, which include 41gt-ag and 1 other. The first FOXP2 report depicted 19 exons, of which two, namely 3a and 3b, are variably spliced into transcripts that cover about 300 kb on the chromosome 7q31 (Lai, Fisher and Hurst).

Several alternative transcripts, as well as protein isoforms, have been predicted on the ...

Section 1: Foundation of the Study

The majority of business information is stored electronically, creating the need for a system of securing this information. As the systems providing computer and internet security becomes more and more complex, security is increasingly compromised. Interlopers and hackers continue to create new and more creative means of gaining entry to information stored on networks, and users are facing an increased number of security attacks. Surprisingly, despite the variety and amount of security risks, security tools designed to counter these are slow to develop appear for business consumption (Garcia-Teodoro et al, 2009). With the introduction of new technological trends, information technology ( ...

The process of DNA replication at biochemical level

DNA or deoxyribonucleic acid is a biopolymer that encodes all the structures needed for the organism’s lifespan and development. In a cell it is located in the nucleus, mitochondria and chloroplasts. DNA consists of four types of deoxyribonucleotides, the sequence of which is used to store the data.

The essential characteristic of DNA molecule that allows it to pass the stored information to the next generations of cells or organisms is the way of self-reproduction known as replication. The molecule consists of two complementary chains that are held together with the help of hydrogen bonds between the complementary bases. ...

DNA (Deoxyribonucleic Acid)

Deoxyribonucleic acid, DNA, is the primary structure that carries the genetic material in cells of living organisms and some viruses. The DNA is made up of strands of nucleotides that are made up of nitrogenous bases, a phosphate compound and, a ribose sugar. The nucleotides form a ring or ladder that over lapses to form double helix strands of polynucleotides. DNA aggregates to form the chromosomes that are strictly found within the nucleus of the cell. Some DNA are found in the ribosomes outside the nucleus. In the DNA structure, the series of the bases is arranged, and the sequence in one ...

Gene expression refers to the mechanism by which information from a gene directs the synthesis of proteins. This process is utilized by eukaryotes and prokaryotes. Gene expression involves various steps. These includes; transcription and translation. The central dogma indicates the flow of genetic information from the Deoxyribonucleic Acid to Ribonucleic Acid and synthesis of the required protein.

Transcription is the production Ribonucleic Acid copies of Deoxyribonucleic acids in the nucleus. The DNA consists of two anti parallel complementary strands, each having 5’ and 3’ ends. The strands serve as a template strand for the production of an RNA transcript. The ...

Deoxyribonucleic acid, DNA, is involved in the manufacturing process of all proteins in the body. It synthesizes proteins that repair and replace worn out tissues in the body. Stem cells (undifferentiated cells with the ability to differentiate) researches have shown that cells from some regions of the body can differentiate into different tissues. Therefore, they can replace worn out tissues and hence function as a repair mechanism. These cells can be induced to become tissue or organ cells. Scientists argue that perhaps the stem cells may carry out similar functions as DNA hence can replace it. This paper seeks to ...

Specific Aims

Activation-induced cytidine deaminase (AID) encodes a DNA-editing deaminase, which is a group of the Cytidine Deaminase family (Bello). This protein is concerned in somatic gene conversion, hypermutation, and class-switch recombination of Ig (immunoglobulin genes). Activation-induced cytidine deaminase (AID) has been expressed in B cells at germinal center and starts the events, which lead to class switch recombination of immunoglobulin genes and somatic hypermutation. Additionally to this primary responsibility in immune diversification, deviant targeting of Activation-induced cytidine deaminase activity leads to translocations and point mutations of oncogenes linked with B-cell lymphoma. Lately, AID has been related in active DNA demethylation (Sariah). ...

The accuracy of the information that health organizations use is a critical asset to the healthcare decision making. The decision made should be based on identification and assessment of appropriate data sources done within the boundary of the data of interest and its availability. The healthcare information is extremely critical as it primary; contains demographic information from admission, medication uses, discharge and treatment of all patients. The health data also contains coronary anatomy and intervention from the laboratory module (Lichtenstein 2004). In addition, health data is obtained directly from the patients, therefore, gives a true reflection the state of the hospital. ...

Lab 1: Ethical Issue in the Movie Gattaca. The epicentre of ethical issues in the movie Gattaca is the manner in which an individual ought to be judged. Should it be by the genetic composition one is born with or by the character traits, personality attributes and ambitions chosen by an individual? Vincent is born with genetic weaknesses that could result in ailments in the future. His genetic composition denies him access to his dream job despite the fact that he achieves the required grades in school and is a strong and athletic young man. As a result, Vincent designs a plan to ...

Abstract

Patients put immense trust in the healthcare providers, and in return expect safety in their hands. Healthcare Informatics, and electronic health records in particular goes a long way in ensuring their safety. The EHR improves care through safer, more accurate and complete information being shared among all the healthcare providers. Wait times for appointments - procedures, and access to community care facilities - are reduced. So are for the laboratory test results and clinical diagnosis. The electronic systems also ensure enhanced security of health information through encryption. It also allows doctors access to secured patients’ information. These include patient physicals, ...

Connective tissue growth factor induces collagen I expression in human lung fibroblasts through the Rac1/MLK3/JNK/AP-1 pathway

Many lung inflammatory diseases present pulmonary fibrosis as one of the symptoms, which is characterized by shortness of breath, chronic coughing and discomfort in the chest. It causes modifications of the lung tissues due to overgrowth, hardening and scarring. This occurs mainly due to deposition of collagen, an extracellular matrix (ECM) protein, whose turnover is regulated by fibroblast. Fibroblast is an important cell regulator that is enlisted by a site of injury. This process is driven by release of transforming growth factor-β (TGF-β), platelet-derived growth factor, interleukin-6 (IL-6), and IL-8/CXCL8. Previously, fibroblast was thought to express no or very ...

Abstract

Human XPA is a protein engaged in DNA damage recognition and repair. A mutation in the gene encoding the protein causes an autosomal recessive disorder, xeroderma pigmentosum (XP), which renders an individual sensitive to sunlight and causes skin cancer. XPA is a 273 amino acid protein, which is predominantly localized in the nucleus. It is expressed at both high and moderate levels in several tissues. The protein serves as a damage sensor in a nucleotide excision repair mechanism, through which the damage to a DNA strand is verified and repaired by formation of a newly synthesized strand. Besides this primary NER mechanism, XPA in conjunction ...

Utilize this template to transcribe a 10 minute segment of your recorded counseling session. Please remember to edit for confidentiality. In addition, you will need to annotate your transcription to identify or highlight a specific skill.

Brief description of Client (age, gender, ethnicity, marital status, employment):

He is a black single man and he’s 23 years old. He is a student in Houston University and he has a roommate. He is family live in different country. He worked a part time and when he needs money in the past but right now he doesn’t have a job.

Brief description of presenting issue:

Alcoholism: Financial Instability due to Alcoholism. The client consumes a lot of alcoholic beverages every day that it ...

The immune system has an immune arsenal that help in the combat of infections including the challenging viral infections (Iannello 2006). Upon infection by a virus, the host invariably reacts by activating its line of defenses. These immune defenses can fall into two main groups: the innate immune system (first line of defense) and adaptive immune response. The innate immune system is non-specific in nature, but the adaptive immune system is acquired following previous infections or vaccinations (Iannello 2006). However, viruses continue to undergo an evolution and have engineered strategies that evade the hosts’ immune antiviral responses. This paper examines ...

(Author)

Regulation of Xiap Protein in Breast Cancer Breast Cancer Breast cancer is the type of cancer that develops in the tissues of the breast. Two most commonly reported types of breast cancer are “ductal carcinoma” and “lobular carcinoma”. Ductal carcinoma is initiated in the lining of the milk ducts, which are thin tubes involved in carrying of milk from lobules of the breast to the nipple. Ductal carcinoma can be in the form of Invasive ductal carcinoma (IDC) as well as Ductal carcinoma in situ (DCIS). IDC is the most commonly found type of breast cancer ...

The Name of the Class (Course)

The Name of the School (University) The City and State Introduction Literature Review According to Geopolymer Institute (2014), Joseph Davidovits discovered Geopolymer concrete. Joseph Davidovits is a French National born in 1935. Geopolymer concrete is as a result of a reaction of source materials. Constituents of source materials are alumina, silica, and alkaline liquid. Geopolymer has a 3-dimensional structure. They are manufactured using low amounts of temperature. The manufacture of Geopolymer involves mixing several products. These products are alkali metal hydroxide and aluminosilicate. Aluminosilicate acts as a binder. It is a constituent of aluminum and silicon. Research shows that Geopolymer ...

Introduction

As a multi-functional protein, Cytoplasmic Actin can be found in all eukaryotic cells at higher concentration levels of over 100 μM. It is the most abundant protein found in most eukaryotic cells, which makes it a critical player in different cellular functions, such as maintaining cell shape and polarity to transcription regulation. The structure of Cytoplasmic Actin is distinguished by a large number of polymorphic proteins which form the filaments. In eukaryotic organisms, such as archaea and eubacteria, actin-like proteins activities are related to sub-cellular organization, motility, cell shape maintenance and cell cycle progression; they are either associated to cell membrane or ...

In the modern society, infectious diseases appear to cause a serious concern. They tend to carry severe economical and healthcare burden, and continue to be responsible for an alarming situation. Infectious disease do evolve in order to become established in a given human host and gradually become responsible for a life threatening situation An early detection and implementation of hygienic protocols is essential to get protection from such conditions. In such context, the present description is concerned with highlighting about HIV virus. Briefly, HIV stands for Human immunodeficiency virus. It is responsible for causing AIDS (Acquired Immunodeficiency Syndrome) in people worldwide. ...

Introduction: Genetic mutations are associated with changes in the genomic sequence that are the main cause of diversity among organisms. The changes in genetic sequence can occur at any instance and are known to have different consequences. Mutations are often associated with a change that affects the nucleic acids in most organisms. Nucleic acids are considered as the building blocks of DNA. On the other hand, DNA are associated with the storage of information that is transferred to the offspring during reproduction. Mutations are associated with diversity and considered as an essential part in evolution. Genetic mutation is considered to ...

Introduction/background

Saccharomyces cerevisiae is an organism that can exist either as a singled-cell organism or as pseudomycelia. It is used in many scientific studies. It is commonly known as baker’s yeast. Saccharomyces cerevisiae is widely employed in scientific studies mainly due to the following reasons: it has a sequenced genome, it is easy to maintain in the lab, researchers can easily manipulate its genetics, and it grows rapidly. Saccharomyces cerevisiae can live with two genomes (diploid, n=32) or one genome (haploid, n=16). Consequently, the organism can undergo meiotic differentiation. In diploid phase, cells of the saccharomyces cerevisiae are more resistant to ...

Properties of Life

When it comes to dealing with cells as a structural and functional unit of all kinds of life on earth, it is important to understand the biological concept of what constitutes life. Most biologists, including cell biologists will agree that life is defined according to seven principles. First, that living things are composed of cells. A cell is a unit that makes up all kinds of life, whether it is a bacterial life or plant life or human life. The second principle is that there are different levels of organization in life – cells make up tissues, tissues make up organs, and so ...

Abstract

Bones are formed through a process known as osteogenesis from three different lineages. This process takes place through two different methods, which are intramembranous ossification and endochondral ossification. A large increment in bone mass takes place in childhood, as well as puberty, through the formation of endochondral bone. At this point, growth hormones are known to play a role. This paper will focus on the process of cartilage and bone formation and the stimulation of bone formation by the growth hormone. There are a number of factors that regulate the formation of bone, while growth hormone affects a number of factors related to ...

Plants emerged before human life in the timeline of evolution. When human beings first evolved from other species, plants were already flourishing in their own kingdom and today, they continue to dominate the earth. In the past, philosophers like Aristotle highlighted the dogmatic view that plants were passive organisms that did not have the ability to sense their environment (Baluška, 2009). Over the centuries, this dogmatic view persisted as human cultures and countries evolved. Darwin was one of the first scientists to talk about dynamic plant life in his book, The Power of the Movement of Plants. Over ...

Embryonic stem cell is a crucial factor in the developmental process of a multicellular organism. Embryonic stem cells (ESCs) are derived at early stages during development and eventually give rise to all type of tissues and organs in an animal. The ES cell lines have three properties consisting of self-renewal, primary chimera formation and pluripotency [1]. Pluripotency, a key feature of ESCs, refers to the capacity of a cell to differentiate into several different types of tissues [2]. An array of cellular factors play important role in maintaining pluripotency and determining cell fate in the developmental pathway. Negative effects on these factors may ...

Learning from My Past: Improving for My Future

“We are products of our past, but we don’t have to be prisoners of it.” Rick Warren As a bilingual student from China, attending college in the United States presented unforeseen challenges as I adjusted to this new environment in America. Even though I was very excited to be here, my ambition did not complement my immaturity. Regrettably, I did not earn straight A’s as done in high school and I did not focus or concentrate enough on my studies as I should, which resulted in earning a C average. Nonetheless, after realizing my errors, I was ...

Question One

Engaging the goals of ideological criticism, provided in To Each Its Own Meaning, and applying this method to the passage of Job 5:1-27 considers the historical, the source, form, tradition-historical, and redaction criticism. Considering the historical ideological criticism of this passage of Job therefore considers the children of Israel 5,000 years ago and the understanding of God the Father as presented in this text. Determining if this passage has an intrinsic ageless value or is it no longer relevant to the contemporary believer remains coherent to the individual. In this, discovering and making visible the dominant ideology(s) ...

“Abstract”

Cholesterol plays a pivotal role in the fluidity of plasma membrane in cells. Evidence of cholesterol evolution from lanosterol suggests that cholesterol has little sensitivity to changes in temperature as opposed to lanosterol. Simulations also revealed that there is no ld-lo coexistence and 3-phase lines in lipid/lanosterol systems as opposed to lipid/cholesterol systems. The sterol also favors a strong interaction with lipids having saturated acyl chains (Miao et al.) which is a key factor in understanding and characterizing the lipid bilayer model. Based on surface potential measurements, cholesterol and ergosterol reduce the penetration of water into the lipid monolayer. Results revealed that excess ...

Most chemotherapy drugs are designed to block DNA replication; majority of the generally and successfully drugs are DNA-damaging agents. The DNA targeting has proven to result in comparatively potent and discerning distribution of cancerous cells. Compounds which are empirically identified with anticancer activity have been indicated to target DNA as the crow flies or through enzyme inhibition which controls the integrity of the DNA of offers the DNA building blocks. With the revelation of the DNA structure various therapeutic modlaities have been established namely; the antimetabolites which, depletes the nucleotides comprising folic acid, an example being methotrexate. Alkylation agents ...

A Brief Review of Epogen

Epogen is a glycoprotein made up of 165 amino acids that is used to stimulate the production of red blood cells. The compound is manufactured through recombinant DNA technology. The compound has a molecular weight equal to 30,400 Daltons. When consumed, its biological effects are similar to those of endogenous erythropoietin. The dugs formulation is a sterile colorless liquid in a buffered solution. It is administered intravenously or subcutaneously. This drug is used by athlete to enhance their performance. This is by producing more red blood cells and opening up lung passages so that more oxygen is delivered to the muscles. Endurance ...

Eukaryotic organisms’ cells are composed of different types of primary and processed RNAs. These RNAs are locally found in the nucleus, cytosol or throughout the cells. To understand the function of RNA as a type of protein, RNA profiling has been carried out leading to the birth of a genomic project known as “ENCODE or the Encyclopedia of DNA Elements”. The goal of the project is to identify and characterize functional elements that are present in the genes (Djebali et al.). In the paper Landscape of Transcription in Human Cells the study tries to answer the general question: should the ...

Question: what does this tell you about this fragment?

The illustration is a 3D structure of E2F4-DNA-DP2 complex. the green fragment is the transcription factor E2F4 protein and it also equates to one out of the four chains that the complete protein has

The fragment functions as a bride between DP-2 and the sequence of DNA. The fragment has the potential of being involved in the process of transcription like functioning as a regulator factor in the cell cycle.

Can you think of a Reason why this stretch is so homologous?

The Yellow fragment corresponds to the DNA-binding domain which is a high homologous sequence of fragment between the E2F series. this portion determines serves as the functional unit of the molecul and ...

The research article "Landscape of transcription in human cells" was a collaborative work done by over 40 researchers. The author’s question is not a direct question, rather, the research paper was a preliminary report of the ENCODE project which is a sort of encyclopedia that contained all the DNA elements in the human genome. The hypothesis was the possibility of producing a catalogue of the whole human genome by first identifying the position and location of the RNAs produced. also, the project also set out to determine whether the 59 termini was modified by the presence of methyl guanosine cap or the ...

“Aboriginal” has since time immemorial been used to describe the indigenous inhabitants of places that were discovered and colonized by European nations. However, the word has of late found widespread use of the term aboriginal in the description of the Australian populace. Before the spread of western civilization most aboriginal languages were entirely spoken languages which did not have any writing system. Before the spread of western civilization, recording of information on written material was not common. This explains why most written aboriginal languages lack voice and identity. However non –aboriginal writers have made large strides in the transcription ...

Phonetic transcriptions illustrate that there are various pronunciation in various accents of English. It is important to note that despite the word being spelled out the same, the fact remains that its pronunciation dictates the region or the culture that any English speaker hails from. The basic spelling of a word cannot be used to explain how a word should be pronounced. The International Phonetic Alphabet (IPA) is a special system that is used in conducting transcription of an English word in different accents. A good example of a phonetic transcription is the word no. According to the IPA phonetic transcription of the ...

of HT22 Cells derived from Mouse Hippocampus

Sections I and II

Biology Section I 1. Introduction Fluorescent protein development research has gained increased knowledge about the range in the color palette available from the Aequorea Victoria jellyfish. The wavelength emissions in the blue to yellow range are now better understood. [1] Another area that has seen a great improvement in knowledge is the understanding of emissions of the monomeric fluorescent protein emissions in the orange to far-red spectral region. [1] The spectral region of fluorescent in the orange to far-red is derived appropriate reef coral species. [1] This area of research ...

Introduction

Scientists have discovered a way of turning off genes so as to provide room for studying how cells work and other aspects of the cells. The discovery has been deemed to be a major success towards a news technology in studying the biochemical pathways and interactions that occur in cells hence facilitating normal development as well as disease progression. Norris (Para. 1) points out that, scientists at UC San Francisco have made this major breakthrough of turning off genes in cells. They indicate that the ability to turn off genes will pave way to increase in research and making of discoveries in ...

Analysis of Culture Cell KU812F with Three Protocols

Abstract Protein analysis for cancer cell research has been found to be extremely reliable. The culture cell KU812F (chronicle myeloid leukemia cell line) was used for this laboratory. Globin gene switching was observed by evaluating the gene expression in the cells. Gene expression and protein folding were provoked. Gene expression is the process of transcribing DNA to cDNA and then used qPCR to study gene expression. cDNA was translated into mRNA which in turn is translated into a protein. mRNA concentration was analyzed. The three protocols were Reverse Transcription Polymerase Chain Reaction (RT-PCR), Enzyme-Linked immune sorbent assay (ELISA), and ...

- Heterogeneous nuclear ribonucleoproteins is a protein that comprises of RNA binding proteins. It is a complex made up of RNA & proteins that is characterized by binding of premRNA. This occurs in the nucleus during the transcription and post transcriptional stages of preMRNA. The protein has different roles which include; prevent formation of secondary structures by preventing folding of pre-mRNA, inhibit the interaction with other proteins, usually has a link with the splicing apparatus and transportation of the mRNA away from the nucleus. This type of protein is a multidomain protein with multiple RNA binding proteins. RNA recognition Motif which is the commonest ...

Genetic testing also known as DNA based test, involves direct examination of DNA molecules to test for genetic disorders and diseases (Gene testing, 2010). According to the Human Genome Project Information (2010), Genetic testing can be used for various reasons to include: - Identifying people who carry one copy of genes causing diseases such as Huntington’s disease. - Pre-symptomatic testing of adult onset diseases/disorders such as Huntington’s disease - Forensic testing and determining paternity. - Newborn screening and pre- natal testing. DNA stands for Deoxyribonucleic acid, and these are chemical Data bases ...

Chloroplast and mitochondria are membrane bound organelles found in eukaryotic cells’ cytoplasm. Chloroplasts are present in all multicellular plants and some algae species while mitochondria are found in most eukaryotic cells. Their main similarity is that both of these organelles generate the universal cell energy carrier known as ATP (Adenosine Triphosphate). - Chloroplast DNA Geneticists have long established that many chloroplast associated traits display cytoplasmic inheritance. This suggests that nuclear genes are not responsible for encoding of these traits. In 1963, it was discovered that chloroplasts have their own deoxyribonucleic acid (DNA) known as chloroplast DNA (cpDNA). ...

Human Immunodeficiency Virus, HIV is a retrovirus, which is a unique virus family consisting of RNA as the genetic material instead of DNA. This genetic material is surrounded by an envelope of lipoprotein. HIV differs structurally from other members of the family retrovirus. It is roughly spherical and has a diameter of around 120 nm. Human Immunodeficiency Virus comprises of two copies of non-covalently joined positive single-stranded RNA enveloped by a conical capsid that consist of the p24 viral protein, which is typical of lentiviruses. Lysine tRNA is reverse transcriptase that is magnesium dependent and is involved in the packaging of both ...

Introduction

Gene expression is the process through which the information that has been encorded in the gene is firstly converted into messenger RNA and then finally to a protein. The study of gene expression is critical especially to the biological student and more so to one who would like to study biology related course like microbiology in future. Therefore, it is important to note that we study gene expression in order to determine how the genes and the proteins which they encode functions in the intact organism. Zhang (2006), Another reason why we study gene expression is in order to ...