Gene replacement refers to a technique which utilizes homologous recombination (Carter & Shieh, 2010, p. 265), to make alterations by either adding a gene, deleting a gene or including point mutations. Gene replacement attempts to cure most of the genetic diseases at molecular level. A successful gene replacement prevents a protein from causing harm, giving new functions to proteins, and recovering or enhancing the normal function of a protein. This technique is commonly used to prevent, treat or improve animals. This is a permanent process that is very beneficial to animals. The process of animal gene replacement involves several processes ...
Essays on Transfection
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Nanog
The paper describes how modern molecular biology methods are applied to identify a new protein called Nanog. They found the sequence of this new protein and determined that it is directly involved in the controlling mechanisms for self-renewal and differentiation of embryonic stem cells. The authors were able to demonstrate that Nanog, as opposed to other previously described proteins that are also involved in self-renewal, works independently an in the absence of external factors like cytokines. Their results also showed that Nanog is not always being produced in the cell; its production is restricted to the events of cell generation. ...