Essays on Weinheim

Introduction

One of the most important part of protein assay is the determination of protein concentration. There are diverse methods used for protein concentration determination, but the most widely used are the spectroscopic methods of analysis or spectroscopy. Spectroscopy usually involves the determination between the correlations of the degree of interaction of light with the analyte, which is the proteins in this particular experiment. There are different types light to analyte interactions and one of these is the absorbance of light or electromagnetic radiation by the analyte. This type of spectrometry is called absorbance spectrometry.

Principle involved in absorbance spectrometry and spectrometers

The majority of substances absorb ...

Abstract

This experiment sort to find an accurate and most viable method that would be used in laboratories to the measurement of iron concentration in aqueous samples. The methodologies that were identified for further analysis included; colorimetric assay (molecular spectroscopy), flame atomic absorption spectroscopy, redox titration (volumetric) and cation exchange chromatography (EMA technique). These methods were analyzed of their advantages and later subjected to a validation test. In the process, a wide range of CRMs were used alongside six other samples that contained unknown iron concentration.

The experiment was thus, geared towards providing answers to statements like; the necessity of quantification of ...